Show simple item record

dc.contributor.authorFulwiler, Carl E.
dc.contributor.authorSchmitt, Ellen A.
dc.contributor.authorKim, Jennie Min-Jung
dc.contributor.authorDowling, John E.
dc.date2022-08-11T08:10:25.000
dc.date.accessioned2022-08-23T17:08:21Z
dc.date.available2022-08-23T17:08:21Z
dc.date.issued1997-05-19
dc.date.submitted2010-02-10
dc.identifier.citationJ Comp Neurol. 1997 May 19;381(4):449-60.
dc.identifier.issn0021-9967 (Linking)
dc.identifier.pmid9136802
dc.identifier.urihttp://hdl.handle.net/20.500.14038/45556
dc.description.abstractDetermination of cell fate in the vertebrate retina has been shown to be largely independent of lineage. After cell fates are determined, retinal neurons become organized in a precise laminar pattern. The mechanisms for this patterning could involve morphogens distributed in gradients or, alternatively, direct cell-cell interactions. In the zebrafish mutant cyclops (Cyc(b16)), most embryos have two partial retinas joined in the ventral midline. This presents developing retinal cells near the midline with abnormal cellular environments, whereas laterally the pattern of developing cells is normal. We examined the consequences of this for patterning in the mutant's retina. We found that the retinas are joined in the midline at the apical surfaces of the photoreceptor layers. A laminar pattern emerges in the midline that preserves normal positional relationships between retinal cell types locally but is abnormal with respect to patterning over the entire retina. Lateral to the midline, retinal patterning appears normal. Metabolic labeling experiments showed that late rounds of DNA synthesis precede the emergence of the novel pattern in this midline region. We conclude that these observations in the cyclops mutant are compatible with mechanisms of pattern formation in the retina involving local cell interactions.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=9136802&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/(SICI)1096-9861(19970519)381:4<449::AID-CNE5>3.0.CO;2-2
dc.subjectAnimals
dc.subjectCell Differentiation
dc.subjectCell Division
dc.subjectDNA
dc.subjectEmbryo, Nonmammalian
dc.subjectHeterozygote
dc.subject*Mutation
dc.subjectNeurons
dc.subjectOptic Nerve
dc.subjectPhenotype
dc.subjectRetina
dc.subjectZebrafish
dc.subjectHealth Services Research
dc.subjectMental and Social Health
dc.subjectPsychiatric and Mental Health
dc.subjectPsychiatry
dc.subjectPsychiatry and Psychology
dc.titleRetinal patterning in the zebrafish mutant cyclops
dc.typeJournal Article
dc.source.journaltitleThe Journal of comparative neurology
dc.source.volume381
dc.source.issue4
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/psych_cmhsr/9
dc.identifier.contextkey1139409
html.description.abstract<p>Determination of cell fate in the vertebrate retina has been shown to be largely independent of lineage. After cell fates are determined, retinal neurons become organized in a precise laminar pattern. The mechanisms for this patterning could involve morphogens distributed in gradients or, alternatively, direct cell-cell interactions. In the zebrafish mutant cyclops (Cyc(b16)), most embryos have two partial retinas joined in the ventral midline. This presents developing retinal cells near the midline with abnormal cellular environments, whereas laterally the pattern of developing cells is normal. We examined the consequences of this for patterning in the mutant's retina. We found that the retinas are joined in the midline at the apical surfaces of the photoreceptor layers. A laminar pattern emerges in the midline that preserves normal positional relationships between retinal cell types locally but is abnormal with respect to patterning over the entire retina. Lateral to the midline, retinal patterning appears normal. Metabolic labeling experiments showed that late rounds of DNA synthesis precede the emergence of the novel pattern in this midline region. We conclude that these observations in the cyclops mutant are compatible with mechanisms of pattern formation in the retina involving local cell interactions.</p>
dc.identifier.submissionpathpsych_cmhsr/9
dc.contributor.departmentDepartment of Psychiatry
dc.source.pages449-60


This item appears in the following Collection(s)

Show simple item record