Confounding effects of volatile anesthesia on CBV assessment in rodent forebrain following ethanol challenge
Treistman, Steven N.
Kim, Young Ro
King, Jean A.
Fox, Gerard B.
Ferris, Craig F.
Document TypeJournal Article
Area Under Curve
Magnetic Resonance Imaging
MetadataShow full item record
AbstractPURPOSE: To compare and contrast the pattern and characteristics of the cerebral blood volume (CBV) response to ethanol (EtOH) in rats under awake and anesthetized conditions. MATERIALS AND METHODS: Acute EtOH (0.75 g/kg) challenge-induced CBV changes were measured using a contrast-enhanced functional MRI CBV method in 15 male Sprague Dawley rats under three experimental conditions: 1.0% to 1.2% isoflurane (N = 5); 0.8% halothane (N = 5); and awake with no anesthetic (N = 5). Physiological parameters were collected from bench settings in nine rats from the above different conditions. Four parameters: 1) area under the curve (AUC%); 2) the maximum signal change (Max%); 3) EtOH absorption rate (alpha(2)); and 4) EtOH elimination rate (alpha(1)) were employed to compare EtOH-induced MRI signals between the awake and anesthetized groups. RESULTS: Both awake and anesthetized animals responded with an increase in CBV to EtOH challenge. However, the presence of anesthesia promoted a significant preferential flow to subcortical areas not seen in the awake condition. CONCLUSION: Unclear mechanisms of anesthesia add a layer of uncertainty to the already complex interpretation of EtOH's influence on neuronal activity, cellular metabolism, and hemodynamic coupling.
SourceJ Magn Reson Imaging. 2007 Sep;26(3):557-63. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/45823
Related ResourcesLink to Article in PubMed