DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity.
| dc.contributor.author | Foyn, Havard | |
| dc.contributor.author | Thompson, Paul R | |
| dc.contributor.author | Arnesen, Thomas | |
| dc.date | 2022-08-11T08:10:32.000 | |
| dc.date.accessioned | 2022-08-23T17:12:03Z | |
| dc.date.available | 2022-08-23T17:12:03Z | |
| dc.date.issued | 2017-03-18 | |
| dc.date.submitted | 2018-12-20 | |
| dc.identifier.citation | <p>Methods Mol Biol. 2017;1574:9-15. doi: 10.1007/978-1-4939-6850-3_2.</p> | |
| dc.identifier.issn | 1940-6029 | |
| dc.identifier.doi | 10.1007/978-1-4939-6850-3_2 | |
| dc.identifier.pmid | 28315240 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14038/46421 | |
| dc.description.abstract | We here describe a quick and easy method to quantitatively measure in vitro acetylation activity of not only N-terminal acetyltransferase (NAT) enzymes, but acetyltransferases using acetyl-coenzyme A as an acetyl donor in general. | |
| dc.language.iso | en_US | |
| dc.publisher | Humana Press | |
| dc.relation.url | https://doi.org/10.1007/978-1-4939-6850-3_2 | |
| dc.subject | Acetyl Coenzyme A | |
| dc.subject | Acetylation | |
| dc.subject | Dithionitrobenzoic Acid | |
| dc.subject | Enzyme Activation | |
| dc.subject | Enzyme Assays | |
| dc.subject | In Vitro Techniques | |
| dc.subject | N-Terminal Acetyltransferases | |
| dc.subject | Substrate Specificity | |
| dc.subject | Acetyl Coenzyme A | |
| dc.subject | Acetylation | |
| dc.subject | Dithionitrobenzoic Acid | |
| dc.subject | Enzyme Activation | |
| dc.subject | Enzyme Assays | |
| dc.subject | In Vitro Techniques | |
| dc.subject | N-Terminal Acetyltransferases | |
| dc.subject | Substrate Specificity | |
| dc.subject | Biochemistry | |
| dc.subject | Enzymes and Coenzymes | |
| dc.subject | Medicinal-Pharmaceutical Chemistry | |
| dc.subject | Molecular Biology | |
| dc.title | DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity. | |
| dc.type | Book Chapter | |
| dc.source.booktitle | Methods in molecular biology (Clifton, N.J.) | |
| dc.source.volume | 1574 | |
| dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/publications/3 | |
| dc.legacy.embargo | 2018-12-20T00:00:00-08:00 | |
| dc.identifier.contextkey | 13518308 | |
| html.description.abstract | <p>We here describe a quick and easy method to quantitatively measure in vitro acetylation activity of not only N-terminal acetyltransferase (NAT) enzymes, but acetyltransferases using acetyl-coenzyme A as an acetyl donor in general.</p> | |
| dc.identifier.submissionpath | publications/3 | |
| dc.contributor.department | Department of Biochemistry and Molecular Pharmacology | |
| dc.contributor.department | Thompson Lab | |
| dc.source.pages | 9-15 |
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