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How accurate is the determination of blood in gastric juice? Comparison of peroxidase and porphyrin methods

dc.contributor.authorWoods, K. L.
dc.contributor.authorKiefe, Catarina I.
dc.contributor.authorGraham, D. Y.
dc.date2022-08-11T08:10:37.000
dc.date.accessioned2022-08-23T17:14:42Z
dc.date.available2022-08-23T17:14:42Z
dc.date.issued1996-06-01
dc.date.submitted2010-04-27
dc.identifier.citationAliment Pharmacol Ther. 1996 Jun;10(3):333-7.
dc.identifier.issn0269-2813 (Linking)
dc.identifier.pmid8791960
dc.identifier.urihttp://hdl.handle.net/20.500.14038/47013
dc.description.abstractBACKGROUND: Mucosal bleeding is frequently used as a measure of gastric mucosal integrity. We compared the orthotolidine method for quantifying haemoglobin in gastric juice with a method based on measurement of fluorescent porphyrins extracted from haem. We also investigated whether acid or pepsin had a deleterious effect on the results of either method. METHODS: We compared the effects of pH (2 or 7), time (up to 180 min), haemoglobin concentration and the addition of pepsin (2.5, 5 or 7.5 kU/mL hog pepsin) on the accuracy of the two methods. RESULTS: With the orthotolidine method there was a time-dependent decline in detectable haemoglobin concentration (P < 0.02) at pH 2 that was not seen at pH 7. The time- and pH-dependent decline in detectable haemoglobin was not seen using the porphyrin assay. CONCLUSION: The widely used orthotolidine method for determination of blood in gastric juice is less reliable than the porphyrin method as it is more likely to be influenced by acidic conditions in the stomach.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=8791960&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1111/j.0953-0673.1996.00333.x
dc.subjectBenzidines
dc.subjectGastric Juice
dc.subjectHemoglobins
dc.subjectHydrogen Peroxide
dc.subjectHydrogen-Ion Concentration
dc.subjectIndicators and Reagents
dc.subjectPepsin A
dc.subjectPeroxidases
dc.subjectPorphyrins
dc.subjectReference Standards
dc.subjectRegression Analysis
dc.subjectReproducibility of Results
dc.subjectBioinformatics
dc.subjectBiostatistics
dc.subjectEpidemiology
dc.subjectHealth Services Research
dc.titleHow accurate is the determination of blood in gastric juice? Comparison of peroxidase and porphyrin methods
dc.typeJournal Article
dc.source.journaltitleAlimentary pharmacology and therapeutics
dc.source.volume10
dc.source.issue3
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/qhs_pp/151
dc.identifier.contextkey1287896
html.description.abstract<p>BACKGROUND: Mucosal bleeding is frequently used as a measure of gastric mucosal integrity. We compared the orthotolidine method for quantifying haemoglobin in gastric juice with a method based on measurement of fluorescent porphyrins extracted from haem. We also investigated whether acid or pepsin had a deleterious effect on the results of either method.</p> <p>METHODS: We compared the effects of pH (2 or 7), time (up to 180 min), haemoglobin concentration and the addition of pepsin (2.5, 5 or 7.5 kU/mL hog pepsin) on the accuracy of the two methods.</p> <p>RESULTS: With the orthotolidine method there was a time-dependent decline in detectable haemoglobin concentration (P < 0.02) at pH 2 that was not seen at pH 7. The time- and pH-dependent decline in detectable haemoglobin was not seen using the porphyrin assay.</p> <p>CONCLUSION: The widely used orthotolidine method for determination of blood in gastric juice is less reliable than the porphyrin method as it is more likely to be influenced by acidic conditions in the stomach.</p>
dc.identifier.submissionpathqhs_pp/151
dc.contributor.departmentDepartment of Quantitative Health Sciences
dc.source.pages333-7


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