Orcutt, Kelly Davis
Hesterman, Jacob Y.
Silva, Matthew D.
UMass Chan AffiliationsDepartment of Radiology
Image Processing, Computer-Assisted
Tomography, Emission-Computed, Single-Photon
Medicinal and Pharmaceutical Chemistry
Medicinal Chemistry and Pharmaceutics
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AbstractRecent advances in small-animal molecular imaging instrumentation combined with well characterized antibody-labeling chemistry have enabled detailed in vivo measurements of antibody distribution in mouse models. This article reviews the strengths and limitations of in vivo antibody imaging methods with a focus on positron emission tomography and single-photon emission computed tomography and a brief discussion of the role of optical imaging in this application. A description of the basic principles behind the imaging techniques is provided along with a discussion of radiolabeling methods relevant to antibodies. Practical considerations of study design and execution are presented through a discussion of sensitivity and resolution tradeoffs for these techniques as defined by modality, signaling probe (isotope or fluorophore) selection, labeling method, and radiation dosimetry. Images and analysis results from a case study are presented with a discussion of output data content and relevant informatics gained with this approach to studying antibody pharmacokinetics.
SourceJ Pharmacol Exp Ther. 2011 May;337(2):350-8. doi: 10.1124/jpet.110.172916. Epub 2011 Feb 11. Link to article on publisher's site.
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/47998
Related ResourcesLink to Article in PubMed