Authors
Hoppin, JackOrcutt, Kelly Davis
Hesterman, Jacob Y.
Silva, Matthew D.
Cheng, Dengfeng
Lackas, Christian
Rusckowski, Mary
UMass Chan Affiliations
Department of RadiologyDocument Type
Journal ArticlePublication Date
2011-05-01Keywords
AnimalsAntibodies
Diagnostic Imaging
Fluorescence
Image Processing, Computer-Assisted
Luminescence
Mice
Pharmacokinetics
Physics
Positron-Emission Tomography
Radiopharmaceuticals
Tomography, Emission-Computed, Single-Photon
Medical Pharmacology
Medicinal and Pharmaceutical Chemistry
Medicinal Chemistry and Pharmaceutics
Radiochemistry
Radiology
Therapeutics
Metadata
Show full item recordAbstract
Recent advances in small-animal molecular imaging instrumentation combined with well characterized antibody-labeling chemistry have enabled detailed in vivo measurements of antibody distribution in mouse models. This article reviews the strengths and limitations of in vivo antibody imaging methods with a focus on positron emission tomography and single-photon emission computed tomography and a brief discussion of the role of optical imaging in this application. A description of the basic principles behind the imaging techniques is provided along with a discussion of radiolabeling methods relevant to antibodies. Practical considerations of study design and execution are presented through a discussion of sensitivity and resolution tradeoffs for these techniques as defined by modality, signaling probe (isotope or fluorophore) selection, labeling method, and radiation dosimetry. Images and analysis results from a case study are presented with a discussion of output data content and relevant informatics gained with this approach to studying antibody pharmacokinetics.Source
J Pharmacol Exp Ther. 2011 May;337(2):350-8. doi: 10.1124/jpet.110.172916. Epub 2011 Feb 11. Link to article on publisher's site.DOI
10.1124/jpet.110.172916Permanent Link to this Item
http://hdl.handle.net/20.500.14038/47998PubMed ID
21317355Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1124/jpet.110.172916