Loss of Arf4 causes severe degeneration of the exocrine pancreas but not cystic kidney disease or retinal degeneration
Authors
Pearring, Jillian N.San Agustin, Jovenal T.
Lobanova, Ekaterina S.
Gabriel, Christopher J.
Lieu, Eric C.
Monis, William J.
Stuck, Michael W.
Strittmatter, Lara
Jaber, Samer M.
Arshavsky, Vadim Y.
Pazour, Gregory J.
UMass Chan Affiliations
Department of Animal MedicineElectron Microscopy Core
Program in Molecular Medicine
Department of Radiology
Document Type
Journal ArticlePublication Date
2017-04-14
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Arf4 is proposed to be a critical regulator of membrane protein trafficking in early secretory pathway. More recently, Arf4 was also implicated in regulating ciliary trafficking, however, this has not been comprehensively tested in vivo. To directly address Arf4's role in ciliary transport, we deleted Arf4 specifically in either rod photoreceptor cells, kidney, or globally during the early postnatal period. Arf4 deletion in photoreceptors did not cause protein mislocalization or retinal degeneration, as expected if Arf4 played a role in protein transport to the ciliary outer segment. Likewise, Arf4 deletion in kidney did not cause cystic disease, as expected if Arf4 were involved in general ciliary trafficking. In contrast, global Arf4 deletion in the early postnatal period resulted in growth restriction, severe pancreatic degeneration and early death. These findings are consistent with Arf4 playing a critical role in endomembrane trafficking, particularly in the pancreas, but not in ciliary function.Source
PLoS Genet. 2017 Apr 14;13(4):e1006740. eCollection 2017 Apr. Link to article on publisher's siteDOI
10.1371/journal.pgen.1006740Permanent Link to this Item
http://hdl.handle.net/20.500.14038/48217PubMed ID
28410364Related Resources
Link to Article in PubMedDistribution License
http://creativecommons.org/licenses/by/4.0/ae974a485f413a2113503eed53cd6c53
10.1371/journal.pgen.1006740
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/