In situ tissue engineering: endothelial growth patterns as a function of flow diverter design
AuthorsMarosfoi, Miklos G.
Langan, Erin T.
van der Marel, Kajo
Lylyk, Ivan R.
Hendricks, Gregory M.
Puri, Ajit S.
Wakhloo, Ajay K.
Gounis, Matthew J.
UMass Chan AffiliationsDepartment of Cell Biology
New England Center for Stroke Research
Department of Radiology
Document TypeJournal Article
Biomedical Devices and Instrumentation
Molecular, Cellular, and Tissue Engineering
MetadataShow full item record
AbstractBACKGROUND: Vascular remodeling in response to implantation of a tissue engineering scaffold such as a flow diverter (FD) leads to the cure of intracranial aneurysms. We hypothesize that the vascular response is dependent on FD design, and CD34+ progenitor cells play an important role in the endothelialization of the implant. METHODS: Sixteen rabbit aneurysms were randomly treated with two different single-layer braided FDs made of cobalt-chrome alloys. The FD-48 and FD-72 devices had 48 and 72 wires, respectively. Aneurysm occlusion rate was assessed during the final digital subtraction angiogram at 10, 20, 30, and 60 days (n=2 per device per time point). Implanted vessels were analyzed with scanning electron microscopy for tissue coverage, endothelialization, and immuno-gold labeling for CD34+ cells. RESULTS: Complete aneurysm occlusion rates were similar between the devices; however, complete or near complete occlusion was more frequently observed in aneurysms with neck < /=4.2 mm (p=0.008). Total tissue coverage at 10 days over the surface of the FD-48 and FD-72 devices was 56.4+/-11.6% and 76.6+/-3.6%, respectively. Endothelial cell growth over the surface was time-dependent for the FD-72 device (Spearman's r=0.86, p=0.013) but not for the FD-48 device (Spearman's r=-0.59, p=0.094). The endothelialization score was marginally correlated with the distance from the aneurysm neck for the FD-48 device (Spearman's r=1, p=0.083) but not for the FD-72 device (Spearman's r=0.8, p=0.33). CD34+ cells were present along the entirety of both devices at all time points. CONCLUSIONS: This study gives preliminary evidence that temporal and spatial endothelialization is dependent on FD design. Circulating CD34+ progenitor cells contribute to endothelialization throughout the healing process.
J Neurointerv Surg. 2017 Oct;9(10):994-998. doi: 10.1136/neurintsurg-2016-012669. Epub 2016 Oct 5. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/48289