Sensors for Proteolytic Activity Visualization and Their Application in Animal Models of Human Diseases
UMass Chan Affiliations
Department of Radiology, Laboratory of Molecular Imaging ProbesDocument Type
Journal ArticlePublication Date
2019-01-01Keywords
sensor moleculesoptical and photo(opto)acoustic imaging
fluorescent signal detection
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Biochemistry
Bioimaging and Biomedical Optics
Chemistry
Enzymes and Coenzymes
Molecular Biology
Radiology
Metadata
Show full item recordAbstract
Various sensors designed for optical and photo(opto)acoustic imaging in living systems are becoming essential components of basic and applied biomedical research. Some of them including those developed for determining enzyme activity in vivo are becoming commercially available. These sensors can be used for various fluorescent signal detection methods: from whole body tomography to endoscopy with miniature cameras. Sensor molecules including enzyme-cleavable macromolecules carrying multiple quenched near-infrared fluorophores are able to deliver their payload in vivo and have long circulation time in bloodstream enabling detection of enzyme activity for extended periods of time at low doses of these sensors. In the future, more effective "activated" probes are expected to become available with optimized sensitivity to enzymatic activity, spectral characteristics suitable for intraoperative imaging of surgical field, biocompatibility and lack of immunogenicity and toxicity. New in vivo optical imaging methods such as the fluorescence lifetime and photo(opto)acoustic imaging will contribute to early diagnosis of human diseases. The use of sensors for in vivo optical imaging will include more extensive preclinical applications of experimental therapies. At the same time, the ongoing development and improvement of optical signal detectors as well as the availability of biologically inert and highly specific fluorescent probes will further contribute to the introduction of fluorescence imaging into the clinic.Source
Biochemistry (Mosc). 2019 Jan;84(Suppl 1):S1-S18. doi: 10.1134/S0006297919140013. Link to article on publisher's site
DOI
10.1134/S0006297919140013Permanent Link to this Item
http://hdl.handle.net/20.500.14038/48372PubMed ID
31213192Related Resources
ae974a485f413a2113503eed53cd6c53
10.1134/S0006297919140013