Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle
Document Type
Journal ArticlePublication Date
2019-12-02Keywords
BiophysicsContraction and Cell Motility
Amino Acids, Peptides, and Proteins
Biophysics
Cell Biology
Cellular and Molecular Physiology
Musculoskeletal System
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Show full item recordAbstract
The thick (myosin-containing) filaments of vertebrate skeletal muscle are arranged in a hexagonal lattice, interleaved with an array of thin (actin-containing) filaments with which they interact to produce contraction. X-ray diffraction and EM have shown that there are two types of thick filament lattice. In the simple lattice, all filaments have the same orientation about their long axis, while in the superlattice, nearest neighbors have rotations differing by 0 degrees or 60 degrees . Tetrapods (amphibians, reptiles, birds, and mammals) typically have only a superlattice, while the simple lattice is confined to fish. We have performed x-ray diffraction and electron microscopy of the soleus (SOL) and extensor digitorum longus (EDL) muscles of the rat and found that while the EDL has a superlattice as expected, the SOL has a simple lattice. The EDL and SOL of the rat are unusual in being essentially pure fast and slow muscles, respectively. The mixed fiber content of most tetrapod muscles and/or lattice disorder may explain why the simple lattice has not been apparent in these vertebrates before. This is supported by only weak simple lattice diffraction in the x-ray pattern of mouse SOL, which has a greater mix of fiber types than rat SOL. We conclude that the simple lattice might be common in tetrapods. The correlation between fiber type and filament lattice arrangement suggests that the lattice arrangement may contribute to the functional properties of a muscle.Source
J Gen Physiol. 2019 Dec 2;151(12):1404-1412. doi: 10.1085/jgp.201912460. Epub 2019 Nov 7. Link to article on publisher's site
DOI
10.1085/jgp.201912460Permanent Link to this Item
http://hdl.handle.net/20.500.14038/48401PubMed ID
31699797Related Resources
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© 2019 Ma et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).Distribution License
http://creativecommons.org/licenses/by-nc-sa/4.0/ae974a485f413a2113503eed53cd6c53
10.1085/jgp.201912460
Scopus Count
Except where otherwise noted, this item's license is described as © 2019 Ma et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).