Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation
Gupta, Rishikesh Kumar
Pappuru, Rajeev Reddy
Hendricks, Gregory M.
Volkert, Michael R.
UMass Chan AffiliationsDepartment of Ophthalmology and Visual Sciences
Department of Microbiology and Physiological Systems
Department of Radiology
Document TypeJournal Article
internal limiting membrane
proliferative diabetic retinopathy
Cellular and Molecular Physiology
MetadataShow full item record
AbstractFibrocellular membrane or epiretinal membrane (ERM) forms on the surface of the inner limiting membrane (ILM) in the inner retina and alters the structure and function of the retina. ERM formation is frequently observed in ocular inflammatory conditions, such as proliferative diabetic retinopathy (PDR) and retinal detachment (RD). Although peeling of the ERM is used as a surgical intervention, it can inadvertently distort the retina. Our goal is to design alternative strategies to tackle ERMs. As a first step, we sought to determine the composition of the ERMs by identifying the constituent cell-types and gene expression signature in patient samples. Using ultrastructural microscopy and immunofluorescence analyses, we found activated microglia, astrocytes, and Muller glia in the ERMs from PDR and RD patients. Moreover, oxidative stress and inflammation associated gene expression was significantly higher in the RD and PDR membranes as compared to the macular hole samples, which are not associated with inflammation. We specifically detected differential expression of hypoxia inducible factor 1-alpha (HIF1-alpha), proinflammatory cytokines, and Notch, Wnt, and ERK signaling pathway-associated genes in the RD and PDR samples. Taken together, our results provide new information to potentially develop methods to tackle ERM formation.
Vishwakarma S, Gupta RK, Jakati S, Tyagi M, Pappuru RR, Reddig K, Hendricks G, Volkert MR, Khanna H, Chhablani J, Kaur I. Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation. Antioxidants (Basel). 2020 Jul 23;9(8):E654. doi: 10.3390/antiox9080654. PMID: 32717933. Link to article on publisher's site