Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation
Authors
Vishwakarma, SushmaGupta, Rishikesh Kumar
Jakati, Saumya
Tyagi, Mudit
Pappuru, Rajeev Reddy
Reddig, Keith
Hendricks, Gregory M.
Volkert, Michael R.
Khanna, Hemant
Chhablani, Jay
Kaur, Inderjeet
UMass Chan Affiliations
Department of Ophthalmology and Visual SciencesDepartment of Microbiology and Physiological Systems
Department of Radiology
Document Type
Journal ArticlePublication Date
2020-07-23Keywords
epiretinal membranehuman retina
internal limiting membrane
macular hole
proliferative diabetic retinopathy
vitreoretinal surgery
Cellular and Molecular Physiology
Eye Diseases
Ophthalmology
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Show full item recordAbstract
Fibrocellular membrane or epiretinal membrane (ERM) forms on the surface of the inner limiting membrane (ILM) in the inner retina and alters the structure and function of the retina. ERM formation is frequently observed in ocular inflammatory conditions, such as proliferative diabetic retinopathy (PDR) and retinal detachment (RD). Although peeling of the ERM is used as a surgical intervention, it can inadvertently distort the retina. Our goal is to design alternative strategies to tackle ERMs. As a first step, we sought to determine the composition of the ERMs by identifying the constituent cell-types and gene expression signature in patient samples. Using ultrastructural microscopy and immunofluorescence analyses, we found activated microglia, astrocytes, and Muller glia in the ERMs from PDR and RD patients. Moreover, oxidative stress and inflammation associated gene expression was significantly higher in the RD and PDR membranes as compared to the macular hole samples, which are not associated with inflammation. We specifically detected differential expression of hypoxia inducible factor 1-alpha (HIF1-alpha), proinflammatory cytokines, and Notch, Wnt, and ERK signaling pathway-associated genes in the RD and PDR samples. Taken together, our results provide new information to potentially develop methods to tackle ERM formation.Source
Vishwakarma S, Gupta RK, Jakati S, Tyagi M, Pappuru RR, Reddig K, Hendricks G, Volkert MR, Khanna H, Chhablani J, Kaur I. Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation. Antioxidants (Basel). 2020 Jul 23;9(8):E654. doi: 10.3390/antiox9080654. PMID: 32717933. Link to article on publisher's site
DOI
10.3390/antiox9080654Permanent Link to this Item
http://hdl.handle.net/20.500.14038/48444PubMed ID
32717933Related Resources
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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Distribution License
http://creativecommons.org/licenses/by/4.0/ae974a485f413a2113503eed53cd6c53
10.3390/antiox9080654
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Except where otherwise noted, this item's license is described as © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).