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dc.contributor.authorVishwakarma, Sushma
dc.contributor.authorGupta, Rishikesh Kumar
dc.contributor.authorJakati, Saumya
dc.contributor.authorTyagi, Mudit
dc.contributor.authorPappuru, Rajeev Reddy
dc.contributor.authorReddig, Keith
dc.contributor.authorHendricks, Gregory M.
dc.contributor.authorVolkert, Michael R.
dc.contributor.authorKhanna, Hemant
dc.contributor.authorChhablani, Jay
dc.contributor.authorKaur, Inderjeet
dc.date2022-08-11T08:10:49.000
dc.date.accessioned2022-08-23T17:21:05Z
dc.date.available2022-08-23T17:21:05Z
dc.date.issued2020-07-23
dc.date.submitted2020-08-27
dc.identifier.citation<p>Vishwakarma S, Gupta RK, Jakati S, Tyagi M, Pappuru RR, Reddig K, Hendricks G, Volkert MR, Khanna H, Chhablani J, Kaur I. Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation. Antioxidants (Basel). 2020 Jul 23;9(8):E654. doi: 10.3390/antiox9080654. PMID: 32717933. <a href="https://doi.org/10.3390/antiox9080654">Link to article on publisher's site</a></p>
dc.identifier.issn2076-3921 (Linking)
dc.identifier.doi10.3390/antiox9080654
dc.identifier.pmid32717933
dc.identifier.urihttp://hdl.handle.net/20.500.14038/48444
dc.description.abstractFibrocellular membrane or epiretinal membrane (ERM) forms on the surface of the inner limiting membrane (ILM) in the inner retina and alters the structure and function of the retina. ERM formation is frequently observed in ocular inflammatory conditions, such as proliferative diabetic retinopathy (PDR) and retinal detachment (RD). Although peeling of the ERM is used as a surgical intervention, it can inadvertently distort the retina. Our goal is to design alternative strategies to tackle ERMs. As a first step, we sought to determine the composition of the ERMs by identifying the constituent cell-types and gene expression signature in patient samples. Using ultrastructural microscopy and immunofluorescence analyses, we found activated microglia, astrocytes, and Muller glia in the ERMs from PDR and RD patients. Moreover, oxidative stress and inflammation associated gene expression was significantly higher in the RD and PDR membranes as compared to the macular hole samples, which are not associated with inflammation. We specifically detected differential expression of hypoxia inducible factor 1-alpha (HIF1-alpha), proinflammatory cytokines, and Notch, Wnt, and ERK signaling pathway-associated genes in the RD and PDR samples. Taken together, our results provide new information to potentially develop methods to tackle ERM formation.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=32717933&dopt=Abstract">Link to Article in PubMed</a></p>
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectepiretinal membrane
dc.subjecthuman retina
dc.subjectinternal limiting membrane
dc.subjectmacular hole
dc.subjectproliferative diabetic retinopathy
dc.subjectvitreoretinal surgery
dc.subjectCellular and Molecular Physiology
dc.subjectEye Diseases
dc.subjectOphthalmology
dc.titleMolecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation
dc.typeJournal Article
dc.source.journaltitleAntioxidants (Basel, Switzerland)
dc.source.volume9
dc.source.issue8
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1562&amp;context=radiology_pubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/radiology_pubs/550
dc.identifier.contextkey19164791
refterms.dateFOA2022-08-23T17:21:05Z
html.description.abstract<p>Fibrocellular membrane or epiretinal membrane (ERM) forms on the surface of the inner limiting membrane (ILM) in the inner retina and alters the structure and function of the retina. ERM formation is frequently observed in ocular inflammatory conditions, such as proliferative diabetic retinopathy (PDR) and retinal detachment (RD). Although peeling of the ERM is used as a surgical intervention, it can inadvertently distort the retina. Our goal is to design alternative strategies to tackle ERMs. As a first step, we sought to determine the composition of the ERMs by identifying the constituent cell-types and gene expression signature in patient samples. Using ultrastructural microscopy and immunofluorescence analyses, we found activated microglia, astrocytes, and Muller glia in the ERMs from PDR and RD patients. Moreover, oxidative stress and inflammation associated gene expression was significantly higher in the RD and PDR membranes as compared to the macular hole samples, which are not associated with inflammation. We specifically detected differential expression of hypoxia inducible factor 1-alpha (HIF1-alpha), proinflammatory cytokines, and Notch, Wnt, and ERK signaling pathway-associated genes in the RD and PDR samples. Taken together, our results provide new information to potentially develop methods to tackle ERM formation.</p>
dc.identifier.submissionpathradiology_pubs/550
dc.contributor.departmentDepartment of Ophthalmology and Visual Sciences
dc.contributor.departmentDepartment of Microbiology and Physiological Systems
dc.contributor.departmentDepartment of Radiology
dc.source.pages654


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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Except where otherwise noted, this item's license is described as © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).