Thermus thermophilus Argonaute Functions in the Completion of DNA Replication
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Authors
Jolly, Samson M.Zhang, Han
Strittmatter, Lara
Bailey, Shannon M.
Hendricks, Gregory M.
Dhabaria, Avantika
Ueberheide, Beatrix
Zamore, Phillip D.
UMass Chan Affiliations
Graduate School of Biomedical SciencesDepartment of Radiology, Division of Cell Biology and Imaging
RNA Therapeutics Institute
Department of Biochemistry and Molecular Pharmacology
Document Type
Journal ArticlePublication Date
2020-09-17Keywords
ArgonauteDNA replication
RNA silencing
Thermus thermophilus
TtAgo
decatenation
gyrase
pAGO
terminus of replication
topoisomerase
Amino Acids, Peptides, and Proteins
Bacteria
Biochemistry
Genetic Phenomena
Genetics and Genomics
Investigative Techniques
Metadata
Show full item recordAbstract
In many eukaryotes, Argonaute proteins, guided by short RNA sequences, defend cells against transposons and viruses. In the eubacterium Thermus thermophilus, the DNA-guided Argonaute TtAgo defends against transformation by DNA plasmids. Here, we report that TtAgo also participates in DNA replication. In vivo, TtAgo binds 15- to 18-nt DNA guides derived from the chromosomal region where replication terminates and associates with proteins known to act in DNA replication. When gyrase, the sole T. thermophilus type II topoisomerase, is inhibited, TtAgo allows the bacterium to finish replicating its circular genome. In contrast, loss of gyrase and TtAgo activity slows growth and produces long sausage-like filaments in which the individual bacteria are linked by DNA. Finally, wild-type T. thermophilus outcompetes an otherwise isogenic strain lacking TtAgo. We propose that the primary role of TtAgo is to help T. thermophilus disentangle the catenated circular chromosomes generated by DNA replication.Source
Jolly SM, Gainetdinov I, Jouravleva K, Zhang H, Strittmatter L, Bailey SM, Hendricks GM, Dhabaria A, Ueberheide B, Zamore PD. Thermus thermophilus Argonaute Functions in the Completion of DNA Replication. Cell. 2020 Sep 17;182(6):1545-1559.e18. doi: 10.1016/j.cell.2020.07.036. Epub 2020 Aug 25. PMID: 32846159; PMCID: PMC7502556. Link to article on publisher's site
DOI
10.1016/j.cell.2020.07.036Permanent Link to this Item
http://hdl.handle.net/20.500.14038/48451PubMed ID
32846159Related Resources
ae974a485f413a2113503eed53cd6c53
10.1016/j.cell.2020.07.036