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dc.contributor.authorKing, Robert M.
dc.contributor.authorCaroff, Jildaz
dc.contributor.authorLangan, Erin T.
dc.contributor.authorLeporati, Anita M.
dc.contributor.authorRodriguez-Rodriguez, Aurora
dc.contributor.authorRaskett, Christopher M.
dc.contributor.authorGupta, Suresh
dc.contributor.authorPuri, Ajit S.
dc.contributor.authorCaravan, Peter
dc.contributor.authorGounis, Matthew J.
dc.contributor.authorBogdanov, Alexei A. Jr.
dc.date2022-08-11T08:10:49.000
dc.date.accessioned2022-08-23T17:21:15Z
dc.date.available2022-08-23T17:21:15Z
dc.date.issued2020-10-05
dc.date.submitted2020-12-17
dc.identifier.citation<p>King RM, Caroff J, Langan ET, Leporati A, Rodriguez-Rodriguez A, Raskett CM, Gupta S, Puri AS, Caravan P, Gounis MJ, Bogdanov AA Jr. In situ decellularization of a large animal saccular aneurysm model: sustained inflammation and active aneurysm wall remodeling. J Neurointerv Surg. 2020 Oct 5:neurintsurg-2020-016589. doi: 10.1136/neurintsurg-2020-016589. Epub ahead of print. PMID: 33020207. <a href="https://doi.org/10.1136/neurintsurg-2020-016589">Link to article on publisher's site</a></p>
dc.identifier.issn1759-8478 (Linking)
dc.identifier.doi10.1136/neurintsurg-2020-016589
dc.identifier.pmid33020207
dc.identifier.urihttp://hdl.handle.net/20.500.14038/48477
dc.description.abstractOBJECTIVE: To investigate in situ decellularization of a large animal model of saccular aneurysm as a strategy for achieving aneurysmal growth and lasting inflammation. METHODS: 18 New Zealand White rabbits were randomized 2:1 to receive endoluminal sodium dodecyl sulfate infusion (SDS, 1% solution, 45 min) following elastase or elastase-only treatment (control). All aneurysms were measured by digital subtraction angiography every 2 weeks. Every 2 weeks, three of the rabbits (two elastase + SDS, one control) underwent MRI, followed by contrast injection with myeloperoxidase (MPO)-sensing contrast agent. MRI was repeated 3 hours after contrast injection and the enhancement ratio (ER) was calculated. Following MRI, aneurysms were explanted and subjected to immunohistopathology. RESULTS: During follow-up MRI, the average ER for SDS-treated animals was 1.63+/-0.20, compared with 1.01+/-0.06 for controls (p < 0.001). The width of SDS-treated aneurysms increased significantly in comparison with the elastase aneurysms (47% vs 20%, p < 0.001). Image analysis of thin sections showed infiltration of MPO-positive cells in decellularized aneurysms and surroundings through the 12-week observation period while control tissue had 5-6 times fewer cells present 2 weeks after aneurysm creation. Immunohistochemistry demonstrated the presence of MPO-positive cells surrounding decellularized lesions at early time points. MPO-positive cells were found in the adventitia and in the thrombi adherent to the aneurysm wall at later time points. CONCLUSIONS: In situ decellularization of a large animal model of saccular aneurysms reproduces features of unstable aneurysms, such as chronic inflammation (up to 12 weeks) and active aneurysm wall remodeling, leading to continued growth over 8 weeks.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=33020207&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1136/neurintsurg-2020-016589
dc.subjectMRI
dc.subjectaneurysm
dc.subjectinflammation
dc.subjectCardiovascular Diseases
dc.subjectNervous System Diseases
dc.subjectNeurology
dc.subjectRadiology
dc.titleIn situ decellularization of a large animal saccular aneurysm model: sustained inflammation and active aneurysm wall remodeling
dc.typeJournal Article
dc.source.journaltitleJournal of neurointerventional surgery
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1592&amp;context=radiology_pubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/radiology_pubs/580
dc.identifier.contextkey20660906
refterms.dateFOA2022-08-23T17:21:15Z
html.description.abstract<p>OBJECTIVE: To investigate in situ decellularization of a large animal model of saccular aneurysm as a strategy for achieving aneurysmal growth and lasting inflammation.</p> <p>METHODS: 18 New Zealand White rabbits were randomized 2:1 to receive endoluminal sodium dodecyl sulfate infusion (SDS, 1% solution, 45 min) following elastase or elastase-only treatment (control). All aneurysms were measured by digital subtraction angiography every 2 weeks. Every 2 weeks, three of the rabbits (two elastase + SDS, one control) underwent MRI, followed by contrast injection with myeloperoxidase (MPO)-sensing contrast agent. MRI was repeated 3 hours after contrast injection and the enhancement ratio (ER) was calculated. Following MRI, aneurysms were explanted and subjected to immunohistopathology.</p> <p>RESULTS: During follow-up MRI, the average ER for SDS-treated animals was 1.63+/-0.20, compared with 1.01+/-0.06 for controls (p < 0.001). The width of SDS-treated aneurysms increased significantly in comparison with the elastase aneurysms (47% vs 20%, p < 0.001). Image analysis of thin sections showed infiltration of MPO-positive cells in decellularized aneurysms and surroundings through the 12-week observation period while control tissue had 5-6 times fewer cells present 2 weeks after aneurysm creation. Immunohistochemistry demonstrated the presence of MPO-positive cells surrounding decellularized lesions at early time points. MPO-positive cells were found in the adventitia and in the thrombi adherent to the aneurysm wall at later time points.</p> <p>CONCLUSIONS: In situ decellularization of a large animal model of saccular aneurysms reproduces features of unstable aneurysms, such as chronic inflammation (up to 12 weeks) and active aneurysm wall remodeling, leading to continued growth over 8 weeks.</p>
dc.identifier.submissionpathradiology_pubs/580
dc.contributor.departmentDepartment of Radiology, Laboratory of Molecular Imaging Probes
dc.contributor.departmentDepartment of Radiology, New England Center for Stroke Research


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