In situ decellularization of a large animal saccular aneurysm model: sustained inflammation and active aneurysm wall remodeling
dc.contributor.author | King, Robert M. | |
dc.contributor.author | Caroff, Jildaz | |
dc.contributor.author | Langan, Erin T. | |
dc.contributor.author | Leporati, Anita M. | |
dc.contributor.author | Rodriguez-Rodriguez, Aurora | |
dc.contributor.author | Raskett, Christopher M. | |
dc.contributor.author | Gupta, Suresh | |
dc.contributor.author | Puri, Ajit S. | |
dc.contributor.author | Caravan, Peter | |
dc.contributor.author | Gounis, Matthew J. | |
dc.contributor.author | Bogdanov, Alexei A. Jr. | |
dc.date | 2022-08-11T08:10:49.000 | |
dc.date.accessioned | 2022-08-23T17:21:15Z | |
dc.date.available | 2022-08-23T17:21:15Z | |
dc.date.issued | 2020-10-05 | |
dc.date.submitted | 2020-12-17 | |
dc.identifier.citation | <p>King RM, Caroff J, Langan ET, Leporati A, Rodriguez-Rodriguez A, Raskett CM, Gupta S, Puri AS, Caravan P, Gounis MJ, Bogdanov AA Jr. In situ decellularization of a large animal saccular aneurysm model: sustained inflammation and active aneurysm wall remodeling. J Neurointerv Surg. 2020 Oct 5:neurintsurg-2020-016589. doi: 10.1136/neurintsurg-2020-016589. Epub ahead of print. PMID: 33020207. <a href="https://doi.org/10.1136/neurintsurg-2020-016589">Link to article on publisher's site</a></p> | |
dc.identifier.issn | 1759-8478 (Linking) | |
dc.identifier.doi | 10.1136/neurintsurg-2020-016589 | |
dc.identifier.pmid | 33020207 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/48477 | |
dc.description.abstract | OBJECTIVE: To investigate in situ decellularization of a large animal model of saccular aneurysm as a strategy for achieving aneurysmal growth and lasting inflammation. METHODS: 18 New Zealand White rabbits were randomized 2:1 to receive endoluminal sodium dodecyl sulfate infusion (SDS, 1% solution, 45 min) following elastase or elastase-only treatment (control). All aneurysms were measured by digital subtraction angiography every 2 weeks. Every 2 weeks, three of the rabbits (two elastase + SDS, one control) underwent MRI, followed by contrast injection with myeloperoxidase (MPO)-sensing contrast agent. MRI was repeated 3 hours after contrast injection and the enhancement ratio (ER) was calculated. Following MRI, aneurysms were explanted and subjected to immunohistopathology. RESULTS: During follow-up MRI, the average ER for SDS-treated animals was 1.63+/-0.20, compared with 1.01+/-0.06 for controls (p < 0.001). The width of SDS-treated aneurysms increased significantly in comparison with the elastase aneurysms (47% vs 20%, p < 0.001). Image analysis of thin sections showed infiltration of MPO-positive cells in decellularized aneurysms and surroundings through the 12-week observation period while control tissue had 5-6 times fewer cells present 2 weeks after aneurysm creation. Immunohistochemistry demonstrated the presence of MPO-positive cells surrounding decellularized lesions at early time points. MPO-positive cells were found in the adventitia and in the thrombi adherent to the aneurysm wall at later time points. CONCLUSIONS: In situ decellularization of a large animal model of saccular aneurysms reproduces features of unstable aneurysms, such as chronic inflammation (up to 12 weeks) and active aneurysm wall remodeling, leading to continued growth over 8 weeks. | |
dc.language.iso | en_US | |
dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=33020207&dopt=Abstract">Link to Article in PubMed</a></p> | |
dc.relation.url | https://doi.org/10.1136/neurintsurg-2020-016589 | |
dc.subject | MRI | |
dc.subject | aneurysm | |
dc.subject | inflammation | |
dc.subject | Cardiovascular Diseases | |
dc.subject | Nervous System Diseases | |
dc.subject | Neurology | |
dc.subject | Radiology | |
dc.title | In situ decellularization of a large animal saccular aneurysm model: sustained inflammation and active aneurysm wall remodeling | |
dc.type | Journal Article | |
dc.source.journaltitle | Journal of neurointerventional surgery | |
dc.identifier.legacyfulltext | https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1592&context=radiology_pubs&unstamped=1 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/radiology_pubs/580 | |
dc.identifier.contextkey | 20660906 | |
refterms.dateFOA | 2022-08-23T17:21:15Z | |
html.description.abstract | <p>OBJECTIVE: To investigate in situ decellularization of a large animal model of saccular aneurysm as a strategy for achieving aneurysmal growth and lasting inflammation.</p> <p>METHODS: 18 New Zealand White rabbits were randomized 2:1 to receive endoluminal sodium dodecyl sulfate infusion (SDS, 1% solution, 45 min) following elastase or elastase-only treatment (control). All aneurysms were measured by digital subtraction angiography every 2 weeks. Every 2 weeks, three of the rabbits (two elastase + SDS, one control) underwent MRI, followed by contrast injection with myeloperoxidase (MPO)-sensing contrast agent. MRI was repeated 3 hours after contrast injection and the enhancement ratio (ER) was calculated. Following MRI, aneurysms were explanted and subjected to immunohistopathology.</p> <p>RESULTS: During follow-up MRI, the average ER for SDS-treated animals was 1.63+/-0.20, compared with 1.01+/-0.06 for controls (p < 0.001). The width of SDS-treated aneurysms increased significantly in comparison with the elastase aneurysms (47% vs 20%, p < 0.001). Image analysis of thin sections showed infiltration of MPO-positive cells in decellularized aneurysms and surroundings through the 12-week observation period while control tissue had 5-6 times fewer cells present 2 weeks after aneurysm creation. Immunohistochemistry demonstrated the presence of MPO-positive cells surrounding decellularized lesions at early time points. MPO-positive cells were found in the adventitia and in the thrombi adherent to the aneurysm wall at later time points.</p> <p>CONCLUSIONS: In situ decellularization of a large animal model of saccular aneurysms reproduces features of unstable aneurysms, such as chronic inflammation (up to 12 weeks) and active aneurysm wall remodeling, leading to continued growth over 8 weeks.</p> | |
dc.identifier.submissionpath | radiology_pubs/580 | |
dc.contributor.department | Department of Radiology, Laboratory of Molecular Imaging Probes | |
dc.contributor.department | Department of Radiology, New England Center for Stroke Research |
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