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    Structural organization of the C1b projection within the ciliary central apparatus

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    Authors
    Cai, Kai
    Zhao, Yanhe
    Zhao, Lei
    Phan, Nhan
    Hou, Yuqing
    Cheng, Xi
    Witman, George B.
    Nicastro, Daniela
    UMass Chan Affiliations
    Division of Cell Biology and Imaging, Department of Radiology
    Document Type
    Journal Article
    Publication Date
    2021-11-01
    Keywords
    Axoneme
    Central pair complex
    Cryo-electron tomography
    Flagella
    Subtomogram averaging
    Amino Acids, Peptides, and Proteins
    Cell Biology
    Cellular and Molecular Physiology
    
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    Link to Full Text
    https://doi.org/10.1242/jcs.254227
    Abstract
    Motile cilia have a '9+2' structure containing nine doublet microtubules and a central apparatus (CA) composed of two singlet microtubules with associated projections. The CA plays crucial roles in regulating ciliary motility. Defects in CA assembly or function usually result in motility-impaired or paralyzed cilia, which in humans causes disease. Despite their importance, the protein composition and functions of most CA projections remain largely unknown. Here, we combined genetic, proteomic and cryo-electron tomographic approaches to compare the CA of wild-type Chlamydomonas reinhardtii with those of three CA mutants. Our results show that two proteins, FAP42 and FAP246, are localized to the L-shaped C1b projection of the CA, where they interact with the candidate CA protein FAP413. FAP42 is a large protein that forms the peripheral 'beam' of the C1b projection, and the FAP246-FAP413 subcomplex serves as the 'bracket' between the beam (FAP42) and the C1b 'pillar' that attaches the projection to the C1 microtubule. The FAP246-FAP413-FAP42 complex is essential for stable assembly of the C1b, C1f and C2b projections, and loss of these proteins leads to ciliary motility defects.
    Source

    Cai K, Zhao Y, Zhao L, Phan N, Hou Y, Cheng X, Witman GB, Nicastro D. Structural organization of the C1b projection within the ciliary central apparatus. J Cell Sci. 2021 Nov 1;134(21):jcs254227. doi: 10.1242/jcs.254227. Epub 2021 Nov 10. PMID: 34651179; PMCID: PMC8627600. Link to article on publisher's site

    DOI
    10.1242/jcs.254227
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/48558
    PubMed ID
    34651179
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    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1242/jcs.254227
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