Authors
Medarova, ZdravkaGreiner, Dale L.
Ifediba, Marytheresa
Dai, Guangping
Bolotin, Elijah
Castillo, Gerardo
Bogdanov, Alexei A. Jr.
Kumar, Mohanraja
Moore, Anna
Document Type
Journal ArticlePublication Date
2011-11-10Keywords
AnimalsDiabetes Mellitus, Experimental
Fluorescein-5-isothiocyanate
Gadolinium DTPA
Magnetic Resonance Imaging
Mice
Pancreas
Rats
Endocrine System
Endocrine System Diseases
Investigative Techniques
Radiology
Metadata
Show full item recordAbstract
BACKGROUND: Vascular parameters, such as vascular volume, flow, and permeability, are important disease biomarkers for both type 1 and type 2 diabetes. Therefore, it is essential to develop approaches to monitor the changes in pancreatic microvasculature non-invasively. METHODS: Here, we describe the application of the long-circulating, paramagnetic T1 contrast agent, protected Graft Copolymer bearing covalently linked gadolinium diethylenetriaminepentaacetic acid residues and labelled with fluorescein (PGC-GdDTPA-F) for the non-invasive semi-quantitative evaluation of vascular changes in diabetic models using magnetic resonance imaging. RESULTS: We observed a significantly higher accumulation of protected graft copolymer bearing covalently linked gadolinium diethylenetriaminepentaacetic acid residues and labelled with fluorescein in the pancreata of BBDR rats induced to develop diabetes, as compared to non-diabetic controls at 1 h post-injection. No differences were seen in the blood pool, kidney, or muscle, indicating that the effect is specific to the diabetic pancreas. Fluorescence microscopy revealed a marked increase in contrast agent availability in the pancreas with the development of the pathology. Similar changes were noted in the homozygous Leprdb mouse model of type 2 diabetes. This effect appeared to result both from the increase of vascular volume and permeability. CONCLUSIONS: High-molecular weight paramagnetic blood volume contrast agents are valuable for the in vivo definition of pancreatic microvasculature dynamics by magnetic resonance imaging. The increase in vascular volume and permeability, associated with diabetic inflammation, can be monitored non-invasively and semi-quantitatively by magnetic resonance imaging in diabetic BBDR rats. This imaging strategy represents a valuable research tool for better understanding of the pathologic process.Source
Diabetes Metab Res Rev. 2011 Nov;27(8):767-72. doi: 10.1002/dmrr.1249. Link to article on publisher's siteDOI
10.1002/dmrr.1249Permanent Link to this Item
http://hdl.handle.net/20.500.14038/48597PubMed ID
22069257Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1002/dmrr.1249