Fluorescent macromolecular sensors of enzymatic activity for in vivo imaging
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UMass Chan Affiliations
Department of RadiologyDocument Type
Book ChapterPublication Date
2013-01-01Keywords
Amino AcidsAnimals
Biosensing Techniques
Carbocyanines
Diagnostic Imaging
Disease Models, Animal
Enzymes
Fluorescence
Fluorescent Dyes
Humans
Macromolecular Substances
Mice
Neoplasm Transplantation
Polyethylene Glycols
Polymers
Spectroscopy, Near-Infrared
Investigative Techniques
Molecular Biology
Radiology
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Show full item recordAbstract
Macromolecular imaging probes (or sensors) of enzymatic activity have a unique place in the armamentarium of modern optical imaging techniques. Such probes were initially developed by attaching optically "silent" fluorophores via enzyme-sensitive linkers to large copolymers of biocompatible poly(ethylene glycol) and poly(amino acids). In diseased tissue, where the concentration of enzymes is high, the fluorophores are freed from the macromolecular carrier and regain their initial ability to fluoresce, thus allowing in vivo optical localization of the diseased tissue. This chapter describes the design and application of these probes and their alternatives in various areas of experimental medicine and gives an overview of currently available techniques that allow imaging of animals using visible and near-infrared light.Source
Prog Mol Biol Transl Sci. 2013;113:349-87. doi: 10.1016/B978-0-12-386932-6.00009-0. Link to article on publisher's siteDOI
10.1016/B978-0-12-386932-6.00009-0Permanent Link to this Item
http://hdl.handle.net/20.500.14038/48611PubMed ID
23244795Notes
Chapter 9 from the book "Fluorescence-Based Biosensors From Concepts to Applications", which is volume 113 in the book series Progress in Molecular Biology and Translational Science.
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Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/B978-0-12-386932-6.00009-0