• Login
    View Item 
    •   Home
    • UMass Chan Departments, Programs and Centers
    • Radiology
    • Radiology Publications
    • View Item
    •   Home
    • UMass Chan Departments, Programs and Centers
    • Radiology
    • Radiology Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of eScholarship@UMassChanCommunitiesPublication DateAuthorsUMass Chan AffiliationsTitlesDocument TypesKeywordsThis CollectionPublication DateAuthorsUMass Chan AffiliationsTitlesDocument TypesKeywords

    My Account

    LoginRegister

    Help

    AboutSubmission GuidelinesData Deposit PolicySearchingAccessibilityTerms of UseWebsite Migration FAQ

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    MR and fluorescence imaging of gadobutrol-induced optical clearing of red fluorescent protein signal in an in vivo cancer model

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Kazachkina, Natalia I.
    Zherdeva, Victoria V.
    Meerovich, Irina G.
    Saydasheva, Asiya N.
    Solovyev, Ilya D.
    Tuchina, Daria K.
    Savitsky, Alexander P.
    Tuchin, Valery V.
    Bogdanov, Alexei A. Jr.
    UMass Chan Affiliations
    Department of Radiology
    Document Type
    Journal Article
    Publication Date
    2022-02-01
    Keywords
    gadobutrol
    multimodality imaging
    optical clearing
    Bioimaging and Biomedical Optics
    Neoplasms
    Radiology
    
    Metadata
    Show full item record
    Link to Full Text
    https://doi.org/10.1002/nbm.4708
    Abstract
    Multimodality registration of optical and MR images in the same tissue volume in vivo may be enabled by MR contrast agents with an optical clearing (OC) effect. The goals of this study were to (a) investigate the effects of clinical MR contrast agent gadobutrol (GB) and its combinations as a potential OC agent assisting in fluorescence intensity (FI) imaging in vivo and (b) evaluate MRI as a tool for imaging of topical or systemic application of GB for the purpose of OC. Subcutaneous tumor xenografts expressing red fluorescent marker protein were used as disease models. MRI was performed at 1 T (1) H MRI using T1 -weighted 3D gradient-echo (T1w-3D GRE) sequences to measure time-dependent MR signal intensity changes by region of interest analysis after image segmentation. Topical application of 1.0 M or 0.7 M GB-containing OC mixture with water and dimethyl sulfoxide showed similar 30-40% increases of tumor FI during the initial 15 min. Afterwards, the OC effect of GB on FI and tumor/background FI ratio showed a decrease over time in the case of 1.0 M GB, unlike the 0.7 M GB mixture, which resulted in a steady increase of FI and tumor/background ratio for 15-60 min. The use of T1w-3D GRE MR pulse sequences showed that concentrated 1.0 M GB resulted in MR signal loss of the skin due to high magnetic susceptibility and that signal loss coincided with the OC effect. Intravenous injection of 0.3 mmol GB/kg resulted in a rapid but transient 40% increase of FI of the tumors. Overall, 1 T MRI enabled tracking of GB-containing OC compositions on the skin surface and tumor tissue, supporting the observation of a time-dependent FI increase in vivo.
    Source

    Kazachkina NI, Zherdeva VV, Meerovich IG, Saydasheva AN, Solovyev ID, Tuchina DK, Savitsky AP, Tuchin VV, Bogdanov AA Jr. MR and fluorescence imaging of gadobutrol-induced optical clearing of red fluorescent protein signal in an in vivo cancer model. NMR Biomed. 2022 Feb 1:e4708. doi: 10.1002/nbm.4708. Epub ahead of print. PMID: 35106848. Link to article on publisher's site

    DOI
    10.1002/nbm.4708
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/48630
    PubMed ID
    35106848
    Related Resources

    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1002/nbm.4708
    Scopus Count
    Collections
    Radiology Publications

    entitlement

    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Lamar Soutter Library, UMass Chan Medical School | 55 Lake Avenue North | Worcester, MA 01655 USA
    Quick Guide | escholarship@umassmed.edu
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.