UMass Chan Affiliations
Department of Cell BiologyDocument Type
Journal ArticlePublication Date
1995-06-11Keywords
AllelesAnimals
Base Sequence
Cells, Cultured
*DNA Transposable Elements
Electroporation
*Genetic Vectors
Molecular Sequence Data
Mutation
*Stem Cells
Cell Biology
Metadata
Show full item recordAbstract
Recombination of an insertion vector into its chromosomal homologue is a conservative event in that both the chromosomal and the vector sequences are preserved. However, gene conversion may accompany homologous recombination of an insertion vector. To examine gene conversion in more detail we have determined the targeting frequencies and the structure of the recombinant alleles generated with a series of vectors which target the hprt gene in embryonic stem cells. We demonstrate that gene conversion of the introduced mutation does not significantly limit homologous recombination and that gene conversion occurs without a sequence specific bias for five different mutations. The frequency of the loss of a vector mutation and the gain of a chromosomal sequence is inversely proportional to the distance between the vector mutation and the double-strand break. The loss of a chromosomal sequence and the gain of a vector mutation occurs at a low frequency.Source
Nucleic Acids Res. 1995 Jun 11;23(11):2058-64. doi: 10.1093/nar/23.11.2058DOI
10.1093/nar/23.11.2058Permanent Link to this Item
http://hdl.handle.net/20.500.14038/48778PubMed ID
7596837Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1093/nar/23.11.2058