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dc.contributor.authorHasty, Paul
dc.contributor.authorRivera-Pérez, Jaime A.
dc.contributor.authorBradley, Allan
dc.date2022-08-11T08:10:51.000
dc.date.accessioned2022-08-23T17:22:35Z
dc.date.available2022-08-23T17:22:35Z
dc.date.issued1995-06-11
dc.date.submitted2011-02-03
dc.identifier.citationNucleic Acids Res. 1995 Jun 11;23(11):2058-64. doi: 10.1093/nar/23.11.2058
dc.identifier.issn0305-1048 (Linking)
dc.identifier.doi10.1093/nar/23.11.2058
dc.identifier.pmid7596837
dc.identifier.urihttp://hdl.handle.net/20.500.14038/48778
dc.description.abstractRecombination of an insertion vector into its chromosomal homologue is a conservative event in that both the chromosomal and the vector sequences are preserved. However, gene conversion may accompany homologous recombination of an insertion vector. To examine gene conversion in more detail we have determined the targeting frequencies and the structure of the recombinant alleles generated with a series of vectors which target the hprt gene in embryonic stem cells. We demonstrate that gene conversion of the introduced mutation does not significantly limit homologous recombination and that gene conversion occurs without a sequence specific bias for five different mutations. The frequency of the loss of a vector mutation and the gain of a chromosomal sequence is inversely proportional to the distance between the vector mutation and the double-strand break. The loss of a chromosomal sequence and the gain of a vector mutation occurs at a low frequency.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=7596837&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectAlleles
dc.subjectAnimals
dc.subjectBase Sequence
dc.subjectCells, Cultured
dc.subject*DNA Transposable Elements
dc.subjectElectroporation
dc.subject*Genetic Vectors
dc.subjectMolecular Sequence Data
dc.subjectMutation
dc.subject*Stem Cells
dc.subjectCell Biology
dc.titleGene conversion during vector insertion in embryonic stem cells
dc.typeArticle
dc.source.journaltitleNucleic acids research
dc.source.volume23
dc.source.issue11
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1013&amp;context=rivera&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/rivera/14
dc.identifier.contextkey1762248
refterms.dateFOA2022-08-23T17:22:35Z
html.description.abstract<p>Recombination of an insertion vector into its chromosomal homologue is a conservative event in that both the chromosomal and the vector sequences are preserved. However, gene conversion may accompany homologous recombination of an insertion vector. To examine gene conversion in more detail we have determined the targeting frequencies and the structure of the recombinant alleles generated with a series of vectors which target the hprt gene in embryonic stem cells. We demonstrate that gene conversion of the introduced mutation does not significantly limit homologous recombination and that gene conversion occurs without a sequence specific bias for five different mutations. The frequency of the loss of a vector mutation and the gain of a chromosomal sequence is inversely proportional to the distance between the vector mutation and the double-strand break. The loss of a chromosomal sequence and the gain of a vector mutation occurs at a low frequency.</p>
dc.identifier.submissionpathrivera/14
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages2058-64


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