The Coding Regions of Germline mRNAs Confer Sensitivity to Argonaute Regulation in C. elegans
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Authors
Seth, MeetuShirayama, Masaki
Tang, Wen
Shen, En-Zhi
Tu, Shikui
Lee, Heng-Chi
Weng, Zhiping
Mello, Craig C.
Document Type
Journal ArticlePublication Date
2018-02-27Keywords
ArgonautesC. elegans
P granules
gene expression
nonsense-mediated decay
perinuclear germline nuage
piRNAs
Biochemistry, Biophysics, and Structural Biology
Cell and Developmental Biology
Genetics and Genomics
Therapeutics
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Show full item recordAbstract
Protein-coding genes undergo a wide array of regulatory interactions with factors that engage non-coding regions. Open reading frames (ORFs), in contrast, are thought to be constrained by coding function, precluding a major role in gene regulation. Here, we explore Piwi-interacting (pi)RNA-mediated transgene silencing in C. elegans and show that marked differences in the sensitivity to piRNA silencing map to the endogenous sequences within transgene ORFs. Artificially increasing piRNA targeting within the ORF of a resistant transgene can lead to a partial yet stable reduction in expression, revealing that piRNAs not only silence but can also "tune" gene expression. Our findings support a model that involves a temporal element to mRNA regulation by germline Argonautes, likely prior to translation, and suggest that piRNAs afford incremental control of germline mRNA expression by targeting the body of the mRNA, including the coding region.Source
Cell Rep. 2018 Feb 27;22(9):2254-2264. doi: 10.1016/j.celrep.2018.02.009. Epub 2018 Feb 15. Link to article on publisher's site
DOI
10.1016/j.celrep.2018.02.009Permanent Link to this Item
http://hdl.handle.net/20.500.14038/48830PubMed ID
29456100Related Resources
Rights
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Distribution License
http://creativecommons.org/licenses/by-nc-nd/4.0/ae974a485f413a2113503eed53cd6c53
10.1016/j.celrep.2018.02.009
Scopus Count
Except where otherwise noted, this item's license is described as This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).