Mechanism of Inhibition of Translation Termination by Blasticidin S
| dc.contributor.author | Svidritskiy, Egor | |
| dc.contributor.author | Korostelev, Andrei A. | |
| dc.date | 2022-08-11T08:10:52.000 | |
| dc.date.accessioned | 2022-08-23T17:22:53Z | |
| dc.date.available | 2022-08-23T17:22:53Z | |
| dc.date.issued | 2018-03-02 | |
| dc.date.submitted | 2019-06-03 | |
| dc.identifier.citation | <p>J Mol Biol. 2018 Mar 2;430(5):591-593. doi: 10.1016/j.jmb.2018.01.007. <a href="https://doi.org/10.1016/j.jmb.2018.01.007">Link to article on publisher's site</a></p> | |
| dc.identifier.issn | 0022-2836 (Linking) | |
| dc.identifier.doi | 10.1016/j.jmb.2018.01.007 | |
| dc.identifier.pmid | 29366636 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14038/48840 | |
| dc.description.abstract | Understanding the mechanisms of inhibitors of translation termination may inform development of new antibacterials and therapeutics for premature termination diseases. We report the crystal structure of the potent termination inhibitor blasticidin S bound to the ribosomal 70S*release factor 1 (RF1) termination complex. Blasticidin S shifts the catalytic domain 3 of RF1 and restructures the peptidyl transferase center. Universally conserved uridine 2585 in the peptidyl transferase center occludes the catalytic backbone of the GGQ motif of RF1, explaining the structural mechanism of inhibition. Rearrangement of domain 3 relative to the codon-recognition domain 2 provides insight into the dynamics of RF1 implicated in termination accuracy. | |
| dc.language.iso | en_US | |
| dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=29366636&dopt=Abstract">Link to Article in PubMed</a></p> | |
| dc.relation.url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831496/ | |
| dc.subject | RF1 | |
| dc.subject | antibiotic | |
| dc.subject | blasticidin S | |
| dc.subject | stop-codon recognition | |
| dc.subject | termination suppression | |
| dc.subject | Biochemistry, Biophysics, and Structural Biology | |
| dc.title | Mechanism of Inhibition of Translation Termination by Blasticidin S | |
| dc.type | Journal Article | |
| dc.source.journaltitle | Journal of molecular biology | |
| dc.source.volume | 430 | |
| dc.source.issue | 5 | |
| dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/rti_pubs/49 | |
| dc.identifier.contextkey | 14647619 | |
| html.description.abstract | <p>Understanding the mechanisms of inhibitors of translation termination may inform development of new antibacterials and therapeutics for premature termination diseases. We report the crystal structure of the potent termination inhibitor blasticidin S bound to the ribosomal 70S*release factor 1 (RF1) termination complex. Blasticidin S shifts the catalytic domain 3 of RF1 and restructures the peptidyl transferase center. Universally conserved uridine 2585 in the peptidyl transferase center occludes the catalytic backbone of the GGQ motif of RF1, explaining the structural mechanism of inhibition. Rearrangement of domain 3 relative to the codon-recognition domain 2 provides insight into the dynamics of RF1 implicated in termination accuracy.</p> | |
| dc.identifier.submissionpath | rti_pubs/49 | |
| dc.contributor.department | RNA Therapeutics Institute | |
| dc.source.pages | 591-593 |
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