CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging
Authors
Ma, HanhuiTu, Li-Chun
Naseri, Ardalan
Chung, Yu-Chieh
Grunwald, David
Zhang, Shaojie
Pederson, Thoru
UMass Chan Affiliations
RNA Therapeutics InstituteDepartment of Biochemistry and Molecular Pharmacology
Document Type
Journal ArticlePublication Date
2018-11-01Keywords
Biochemistry, Biophysics, and Structural BiologyBioimaging and Biomedical Optics
Genetics and Genomics
Metadata
Show full item recordAbstract
Clustered regularly interspaced short palindromic repeats (CRISPR) guide RNA scaffolds have been adapted to carry multiple binding sites for fluorescent proteins to enhance brightness for live cell imaging of genomic loci. However, many of these modifications result in guide RNA instability and thus produce lower genome-labeling efficiency than anticipated. Here we introduce CRISPR-Sirius, based on octet arrays of aptamers conferring both enhanced guide RNA stability and brightness, and provide initial biological applications of this platform.Source
Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30. Link to article on publisher's site
DOI
10.1038/s41592-018-0174-0Permanent Link to this Item
http://hdl.handle.net/20.500.14038/48850PubMed ID
30377374Related Resources
ae974a485f413a2113503eed53cd6c53
10.1038/s41592-018-0174-0