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    CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging

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    Authors
    Ma, Hanhui
    Tu, Li-Chun
    Naseri, Ardalan
    Chung, Yu-Chieh
    Grunwald, David
    Zhang, Shaojie
    Pederson, Thoru
    UMass Chan Affiliations
    RNA Therapeutics Institute
    Department of Biochemistry and Molecular Pharmacology
    Document Type
    Journal Article
    Publication Date
    2018-11-01
    Keywords
    Biochemistry, Biophysics, and Structural Biology
    Bioimaging and Biomedical Optics
    Genetics and Genomics
    
    Metadata
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    Link to Full Text
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252086/
    Abstract
    Clustered regularly interspaced short palindromic repeats (CRISPR) guide RNA scaffolds have been adapted to carry multiple binding sites for fluorescent proteins to enhance brightness for live cell imaging of genomic loci. However, many of these modifications result in guide RNA instability and thus produce lower genome-labeling efficiency than anticipated. Here we introduce CRISPR-Sirius, based on octet arrays of aptamers conferring both enhanced guide RNA stability and brightness, and provide initial biological applications of this platform.
    Source

    Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30. Link to article on publisher's site

    DOI
    10.1038/s41592-018-0174-0
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/48850
    PubMed ID
    30377374
    Related Resources

    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1038/s41592-018-0174-0
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