CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging
| dc.contributor.author | Ma, Hanhui | |
| dc.contributor.author | Tu, Li-Chun | |
| dc.contributor.author | Naseri, Ardalan | |
| dc.contributor.author | Chung, Yu-Chieh | |
| dc.contributor.author | Grunwald, David | |
| dc.contributor.author | Zhang, Shaojie | |
| dc.contributor.author | Pederson, Thoru | |
| dc.date | 2022-08-11T08:10:52.000 | |
| dc.date.accessioned | 2022-08-23T17:22:56Z | |
| dc.date.available | 2022-08-23T17:22:56Z | |
| dc.date.issued | 2018-11-01 | |
| dc.date.submitted | 2019-09-18 | |
| dc.identifier.citation | <p>Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30. <a href="https://doi.org/10.1038/s41592-018-0174-0">Link to article on publisher's site</a></p> | |
| dc.identifier.issn | 1548-7091 (Linking) | |
| dc.identifier.doi | 10.1038/s41592-018-0174-0 | |
| dc.identifier.pmid | 30377374 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14038/48850 | |
| dc.description.abstract | Clustered regularly interspaced short palindromic repeats (CRISPR) guide RNA scaffolds have been adapted to carry multiple binding sites for fluorescent proteins to enhance brightness for live cell imaging of genomic loci. However, many of these modifications result in guide RNA instability and thus produce lower genome-labeling efficiency than anticipated. Here we introduce CRISPR-Sirius, based on octet arrays of aptamers conferring both enhanced guide RNA stability and brightness, and provide initial biological applications of this platform. | |
| dc.language.iso | en_US | |
| dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=30377374&dopt=Abstract">Link to Article in PubMed</a></p> | |
| dc.relation.url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252086/ | |
| dc.subject | Biochemistry, Biophysics, and Structural Biology | |
| dc.subject | Bioimaging and Biomedical Optics | |
| dc.subject | Genetics and Genomics | |
| dc.title | CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging | |
| dc.type | Journal Article | |
| dc.source.journaltitle | Nature methods | |
| dc.source.volume | 15 | |
| dc.source.issue | 11 | |
| dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/rti_pubs/59 | |
| dc.identifier.contextkey | 15358659 | |
| html.description.abstract | <p>Clustered regularly interspaced short palindromic repeats (CRISPR) guide RNA scaffolds have been adapted to carry multiple binding sites for fluorescent proteins to enhance brightness for live cell imaging of genomic loci. However, many of these modifications result in guide RNA instability and thus produce lower genome-labeling efficiency than anticipated. Here we introduce CRISPR-Sirius, based on octet arrays of aptamers conferring both enhanced guide RNA stability and brightness, and provide initial biological applications of this platform.</p> | |
| dc.identifier.submissionpath | rti_pubs/59 | |
| dc.contributor.department | RNA Therapeutics Institute | |
| dc.contributor.department | Department of Biochemistry and Molecular Pharmacology | |
| dc.source.pages | 928-931 |