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    Repeated cleavage failure does not establish centrosome amplification in untransformed human cells

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    Authors
    Krzywicka-Racka, Anna
    Sluder, Greenfield
    UMass Chan Affiliations
    Department of Cell Biology
    Document Type
    Journal Article
    Publication Date
    2011-07-25
    Keywords
    Animals
    CHO Cells
    Cell Proliferation
    Cells, Cultured
    Centrosome
    Cricetinae
    Cricetulus
    HeLa Cells
    Humans
    Immunohistochemistry
    Tumor Suppressor Protein p53
    Cell Biology
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    Abstract
    We tested whether cleavage failure as a transient event establishes an incidence of centrosome amplification in cell populations. Five rounds of approximately 30% cytochalasin-induced cleavage failure in untransformed human cell cultures did not establish centrosome amplification in the short or long terms. The progeny of binucleate cells progressively dropped out of the cell cycle and expressed p53/p21, and none divided a fourth time. We also tested whether cleavage failure established centrosome amplification in transformed cell populations. Tetraploid HCT116 p53(-/-) cells eventually all failed cleavage repeatedly and ceased proliferating. HeLa cells all died or arrested within four cell cycles. Chinese hamster ovary cells proliferated after cleavage failure, but five rounds of induced cleavage failure produced a modest increase in the incidence of centrosome amplification in the short term, which did not rise with more cycles of cleavage failure. This incidence dropped to close to control values in the long term despite a 2-6% rate of spontaneous cleavage failure in the progeny of tetraploid cells.
    Source
    J Cell Biol. 2011 Jul 25;194(2):199-207. Link to article on publisher's site
    DOI
    10.1083/jcb.201101073
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/49023
    PubMed ID
    21788368
    Related Resources
    Link to Article in PubMed
    Rights

    © 2011 Krzywicka-Racka and Sluder. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

    ae974a485f413a2113503eed53cd6c53
    10.1083/jcb.201101073
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