We are upgrading the repository! A content freeze is in effect until December 6, 2024. New submissions or changes to existing items will not be allowed during this period. All content already published will remain publicly available for searching and downloading. Updates will be posted in the Website Upgrade 2024 FAQ in the sidebar Help menu. Reach out to escholarship@umassmed.edu with any questions.
Magnetic resonance imaging of tissue and vascular layers in the cat retina
Authors
Shen, QiangCheng, Haiying
Pardue, Machelle T.
Chang, Thomas F.
Nair, Govind
Vo, Van Toi
Shonat, Ross D.
Duong, Timothy Q.
Faculty Advisor
Timothy Q. DuongUMass Chan Affiliations
T.H. Chan School of MedicineDocument Type
Journal ArticlePublication Date
2006-04-01Keywords
AnimalsCats
Contrast Media
Diffusion Magnetic Resonance Imaging
Female
Gadolinium DTPA
Image Processing, Computer-Assisted
Magnetic Resonance Imaging
Retina
Subtraction Technique
Radiology
Metadata
Show full item recordAbstract
PURPOSE: To report the visual resolution of multiple cell and vascular "layers" in the cat retina using MRI. MATERIALS AND METHODS: T2- and diffusion-weighted MRI at 4.7 Tesla was performed. Layer-specific thickness, T2, spin density, apparent diffusion coefficient perpendicular (ADC(perpendicular)) and parallel (ADC(parallel)) to the retinal surface were tabulated. T1-weighted MRI was acquired before and after intravenous administration of Gd-DTPA and subtraction images were obtained. Histology was performed for validation. RESULTS: Three distinct "layers" were observed. The inner strip nearest to the vitreous (exhibiting large T2, ADC, spin density with Gd-DTPA enhancement) overlapped the ganglion cell layer, bipolar cell layer, and the embedded retinal vascular layer. The middle strip (exhibiting small T2, ADC, spin density without Gd-DTPA enhancement) overlapped the photoreceptor cell layer and the inner and outer segments. The outer strip (exhibiting large T2, ADC, spin density with Gd-DTPA enhancement) overlapped the tapetum and choroidal vascular layer. T2, spin density, ADC(perpendicular) and ADC(parallel) of different "layers" were tabulated. The inner strip was slightly thicker than the other two strips. The total thickness, including neural and nonneural retina, was 358 +/- 13 microm (N = 6) by MRI and 319 +/- 77 microm (N = 5) by histology. CONCLUSION: MRI provides a noninvasive tool to study the retina with laminar specificity without depth limitation.Source
J Magn Reson Imaging. 2006 Apr;23(4):465-72. Link to article on publisher's siteDOI
10.1002/jmri.20549Permanent Link to this Item
http://hdl.handle.net/20.500.14038/49236PubMed ID
16523482Notes
Medical student Thomas F. Chang participated in this study as part of the Senior Scholars research program at the University of Massachusetts Medical School.
Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1002/jmri.20549