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dc.contributor.advisorCharles Vacanti
dc.contributor.authorRodriguez, Angela
dc.contributor.authorCao, Yi Lin
dc.contributor.authorIbarra, Clemente
dc.contributor.authorPap, Stephen
dc.contributor.authorVacanti, Martin
dc.contributor.authorEavey, Roland D.
dc.contributor.authorVacanti, Charles A.
dc.date2022-08-11T08:10:56.000
dc.date.accessioned2022-08-23T17:25:34Z
dc.date.available2022-08-23T17:25:34Z
dc.date.issued1999-03-24
dc.date.submitted2010-01-27
dc.identifier.citation<p>Plast Reconstr Surg. 1999 Apr;103(4):1111-9.</p>
dc.identifier.issn0032-1052 (Linking)
dc.identifier.doi10.1097/00006534-199904040-00001
dc.identifier.pmid10088494
dc.identifier.urihttp://hdl.handle.net/20.500.14038/49435
dc.description<p>Medical student Stephen Pap participated in this study as part of the Senior Scholars research program.</p>
dc.description.abstractIn the repair of cartilage defects, autologous tissue offers the advantage of lasting biocompatibility. The ability of bovine chondrocytes isolated from hyaline cartilage to generate tissue-engineered cartilage in a predetermined shape, such as a human ear, has been demonstrated; however, the potential of chondrocytes isolated from human elastic cartilage remains unknown. In this study, the authors examined the multiplication characteristics of human auricular chondrocytes and the ability of these cells to generate new elastic cartilage as a function of the length of time they are maintained in vitro. Human auricular cartilage, harvested from patients 5 to 17 years of age, was digested in collagenase, and the chondrocytes were isolated and cultured in vitro for up to 12 weeks. Cells were trypsinized, counted, and passaged every 2 weeks. Chondrocyte-polymer (polyglycolic acid) constructs were created at each passage and then implanted into athymic mice for 8 weeks. The ability of the cells to multiply in vitro and their ability to generate new cartilage as a function of the time they had been maintained in vitro were studied. A total of 31 experimental constructs from 12 patients were implanted and compared with a control group of constructs without chondrocytes. In parallel, a representative sample of cells was evaluated to determine the presence of collagen. The doubling rate of human auricular chondrocytes in vitro remained constant within the population studied. New tissue developed in 22 of 31 experimental implants. This tissue demonstrated the physical characteristics of auricular cartilage on gross inspection. Histologically, specimens exhibited dense cellularity and lacunae-containing cells embedded in a basophilic matrix. The specimens resembled immature cartilage and were partially devoid of the synthetic material of which the construct had been composed. Analyses for collagen, proteoglycans, and elastin were consistent with elastic cartilage. No cartilage was detected in the control implants. Human auricular chondrocytes multiply well in vitro and possess the ability to form new cartilage when seeded onto a three-dimensional scaffold. These growth characteristics might some day enable chondrocytes isolated from a small auricular biopsy to be expanded in vitro to generate a large, custom-shaped, autologous graft for clinical reconstruction of a cartilage defect, such as for congenital microtia.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=10088494&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1097/00006534-199904040-00001
dc.subjectAbsorbable Implants
dc.subjectAdolescent
dc.subjectAnimals
dc.subjectCell Differentiation
dc.subjectCell Division
dc.subjectCells, Cultured
dc.subjectChild
dc.subjectChondrocytes
dc.subjectCollagen
dc.subjectEar Cartilage
dc.subjectElastin
dc.subjectHumans
dc.subjectMale
dc.subjectMice
dc.subjectMice, Nude
dc.subjectPhotomicrography
dc.subjectPolyglycolic Acid
dc.subjectProteoglycans
dc.subjectReconstructive Surgical Procedures
dc.subjectTime Factors
dc.subjectPlastic Surgery
dc.subjectSurgery
dc.titleCharacteristics of cartilage engineered from human pediatric auricular cartilage
dc.typeJournal Article
dc.source.journaltitlePlastic and reconstructive surgery
dc.source.volume103
dc.source.issue4
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/ssp/91
dc.legacy.embargo1999-01-01T00:00:00-08:00
dc.identifier.contextkey1123100
html.description.abstract<p>In the repair of cartilage defects, autologous tissue offers the advantage of lasting biocompatibility. The ability of bovine chondrocytes isolated from hyaline cartilage to generate tissue-engineered cartilage in a predetermined shape, such as a human ear, has been demonstrated; however, the potential of chondrocytes isolated from human elastic cartilage remains unknown. In this study, the authors examined the multiplication characteristics of human auricular chondrocytes and the ability of these cells to generate new elastic cartilage as a function of the length of time they are maintained in vitro. Human auricular cartilage, harvested from patients 5 to 17 years of age, was digested in collagenase, and the chondrocytes were isolated and cultured in vitro for up to 12 weeks. Cells were trypsinized, counted, and passaged every 2 weeks. Chondrocyte-polymer (polyglycolic acid) constructs were created at each passage and then implanted into athymic mice for 8 weeks. The ability of the cells to multiply in vitro and their ability to generate new cartilage as a function of the time they had been maintained in vitro were studied. A total of 31 experimental constructs from 12 patients were implanted and compared with a control group of constructs without chondrocytes. In parallel, a representative sample of cells was evaluated to determine the presence of collagen. The doubling rate of human auricular chondrocytes in vitro remained constant within the population studied. New tissue developed in 22 of 31 experimental implants. This tissue demonstrated the physical characteristics of auricular cartilage on gross inspection. Histologically, specimens exhibited dense cellularity and lacunae-containing cells embedded in a basophilic matrix. The specimens resembled immature cartilage and were partially devoid of the synthetic material of which the construct had been composed. Analyses for collagen, proteoglycans, and elastin were consistent with elastic cartilage. No cartilage was detected in the control implants. Human auricular chondrocytes multiply well in vitro and possess the ability to form new cartilage when seeded onto a three-dimensional scaffold. These growth characteristics might some day enable chondrocytes isolated from a small auricular biopsy to be expanded in vitro to generate a large, custom-shaped, autologous graft for clinical reconstruction of a cartilage defect, such as for congenital microtia.</p>
dc.identifier.submissionpathssp/91
dc.contributor.departmentDepartment of Anesthesiology
dc.source.pages1111-9


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