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Nuclear antigens in the HeLa cell cycle

dc.contributor.authorBanjar, Zainy M.
dc.contributor.authorBriggs, Robert C.
dc.contributor.authorHnilica, Lobumir S.
dc.contributor.authorStein, Janet L.
dc.contributor.authorStein, Gary S.
dc.date2022-08-11T08:10:56.000
dc.date.accessioned2022-08-23T17:25:47Z
dc.date.available2022-08-23T17:25:47Z
dc.date.issued1985-07-01
dc.date.submitted2011-01-14
dc.identifier.citationMol Cell Biochem. 1985 Jul;67(2):101-10.
dc.identifier.issn0300-8177 (Linking)
dc.identifier.pmid4047024
dc.identifier.urihttp://hdl.handle.net/20.500.14038/49486
dc.description.abstractAntisera to 0.35 M NaCl extracts and residues of S phase HeLa nuclei were reacted with electrophoretically separated proteins from the nuclei or nuclear material of HeLa cells synchronized in G1, S, G2 or M phases of the cell cycle. Quantitative evaluation of the peroxidase-antiperoxidase stained nitrocellulose transfers (Western blots) revealed significant changes in the quantities of nuclear non-histone proteins during the cell cycle. Immunochemical staining of electrophoretically separated nuclear antigens permits their selective detection in minute quantities and in the presence of many additional proteins.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=4047024&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1007/BF02370168
dc.subjectAntigens
dc.subjectAntigens, Nuclear
dc.subjectCell Cycle
dc.subjectHela Cells
dc.subjectHumans
dc.subjectInterphase
dc.subjectMetaphase
dc.subjectNucleoproteins
dc.subjectCell Biology
dc.titleNuclear antigens in the HeLa cell cycle
dc.typeJournal Article
dc.source.journaltitleMolecular and cellular biochemistry
dc.source.volume67
dc.source.issue2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/stein/142
dc.identifier.contextkey1728447
html.description.abstract<p>Antisera to 0.35 M NaCl extracts and residues of S phase HeLa nuclei were reacted with electrophoretically separated proteins from the nuclei or nuclear material of HeLa cells synchronized in G1, S, G2 or M phases of the cell cycle. Quantitative evaluation of the peroxidase-antiperoxidase stained nitrocellulose transfers (Western blots) revealed significant changes in the quantities of nuclear non-histone proteins during the cell cycle. Immunochemical staining of electrophoretically separated nuclear antigens permits their selective detection in minute quantities and in the presence of many additional proteins.</p>
dc.identifier.submissionpathstein/142
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages101-10


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