Synthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S3 cells
| dc.contributor.author | Detke, Siegfried | |
| dc.contributor.author | Stein, Janet L. | |
| dc.contributor.author | Stein, Gary S. | |
| dc.date | 2022-08-11T08:10:56.000 | |
| dc.date.accessioned | 2022-08-23T17:25:55Z | |
| dc.date.available | 2022-08-23T17:25:55Z | |
| dc.date.issued | 1978-05-01 | |
| dc.date.submitted | 2011-01-14 | |
| dc.identifier.citation | Nucleic Acids Res. 1978 May;5(5):1515-28. <a href="http://dx.doi.org/10.1093/nar/5.5.1515">Link to article on publisher's website</a> | |
| dc.identifier.issn | 0305-1048 (Linking) | |
| dc.identifier.doi | 10.1093/nar/5.5.1515 | |
| dc.identifier.pmid | 662692 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14038/49516 | |
| dc.description.abstract | Nuclei were isolated from synchronized HeLa S3 cells and transcribed utilizing their endogenous RNA polymerases. Our data suggest that S phase nuclei are capable of synthesizing histone mRNA sequences while nuclei from G1 phase cells are not. Transcription of histone mRNA sequences by S phase nuclei can be abolished completely by low levels of alpha-amanitin (1.0 microgram/ml, a concentration which completely inhibits RNA polymerase II). From these results it appears that transcription of the histone mRNA sequences occurs during the S phase but not during the G1 phase of the cell cycle and that RNA polymerase II is responsible for histone gene readout. | |
| dc.language.iso | en_US | |
| dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=662692&dopt=Abstract">Link to Article in PubMed</a> | |
| dc.subject | Amanitins | |
| dc.subject | Cell Cycle | |
| dc.subject | Cell Nucleus | |
| dc.subject | DNA-Directed RNA Polymerases | |
| dc.subject | Hela Cells | |
| dc.subject | Histones | |
| dc.subject | Kinetics | |
| dc.subject | Nucleic Acid Hybridization | |
| dc.subject | RNA Polymerase II | |
| dc.subject | RNA, Messenger | |
| dc.subject | Transcription, Genetic | |
| dc.subject | Cell Biology | |
| dc.title | Synthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S3 cells | |
| dc.type | Journal Article | |
| dc.source.journaltitle | Nucleic acids research | |
| dc.source.volume | 5 | |
| dc.source.issue | 5 | |
| dc.identifier.legacyfulltext | https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1181&context=stein&unstamped=1 | |
| dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/stein/182 | |
| dc.identifier.contextkey | 1728487 | |
| refterms.dateFOA | 2022-08-23T17:25:55Z | |
| html.description.abstract | <p>Nuclei were isolated from synchronized HeLa S3 cells and transcribed utilizing their endogenous RNA polymerases. Our data suggest that S phase nuclei are capable of synthesizing histone mRNA sequences while nuclei from G1 phase cells are not. Transcription of histone mRNA sequences by S phase nuclei can be abolished completely by low levels of alpha-amanitin (1.0 microgram/ml, a concentration which completely inhibits RNA polymerase II). From these results it appears that transcription of the histone mRNA sequences occurs during the S phase but not during the G1 phase of the cell cycle and that RNA polymerase II is responsible for histone gene readout.</p> | |
| dc.identifier.submissionpath | stein/182 | |
| dc.contributor.department | Department of Cell Biology | |
| dc.source.pages | 1515-28 |
