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dc.contributor.authorDetke, Siegfried
dc.contributor.authorStein, Janet L.
dc.contributor.authorStein, Gary S.
dc.date2022-08-11T08:10:56.000
dc.date.accessioned2022-08-23T17:25:55Z
dc.date.available2022-08-23T17:25:55Z
dc.date.issued1978-05-01
dc.date.submitted2011-01-14
dc.identifier.citationNucleic Acids Res. 1978 May;5(5):1515-28. <a href="http://dx.doi.org/10.1093/nar/5.5.1515">Link to article on publisher's website</a>
dc.identifier.issn0305-1048 (Linking)
dc.identifier.doi10.1093/nar/5.5.1515
dc.identifier.pmid662692
dc.identifier.urihttp://hdl.handle.net/20.500.14038/49516
dc.description.abstractNuclei were isolated from synchronized HeLa S3 cells and transcribed utilizing their endogenous RNA polymerases. Our data suggest that S phase nuclei are capable of synthesizing histone mRNA sequences while nuclei from G1 phase cells are not. Transcription of histone mRNA sequences by S phase nuclei can be abolished completely by low levels of alpha-amanitin (1.0 microgram/ml, a concentration which completely inhibits RNA polymerase II). From these results it appears that transcription of the histone mRNA sequences occurs during the S phase but not during the G1 phase of the cell cycle and that RNA polymerase II is responsible for histone gene readout.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=662692&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectAmanitins
dc.subjectCell Cycle
dc.subjectCell Nucleus
dc.subjectDNA-Directed RNA Polymerases
dc.subjectHela Cells
dc.subjectHistones
dc.subjectKinetics
dc.subjectNucleic Acid Hybridization
dc.subjectRNA Polymerase II
dc.subjectRNA, Messenger
dc.subjectTranscription, Genetic
dc.subjectCell Biology
dc.titleSynthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S3 cells
dc.typeJournal Article
dc.source.journaltitleNucleic acids research
dc.source.volume5
dc.source.issue5
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1181&amp;context=stein&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/stein/182
dc.identifier.contextkey1728487
refterms.dateFOA2022-08-23T17:25:55Z
html.description.abstract<p>Nuclei were isolated from synchronized HeLa S3 cells and transcribed utilizing their endogenous RNA polymerases. Our data suggest that S phase nuclei are capable of synthesizing histone mRNA sequences while nuclei from G1 phase cells are not. Transcription of histone mRNA sequences by S phase nuclei can be abolished completely by low levels of alpha-amanitin (1.0 microgram/ml, a concentration which completely inhibits RNA polymerase II). From these results it appears that transcription of the histone mRNA sequences occurs during the S phase but not during the G1 phase of the cell cycle and that RNA polymerase II is responsible for histone gene readout.</p>
dc.identifier.submissionpathstein/182
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages1515-28


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