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dc.contributor.authorStein, Janet L.
dc.contributor.authorStein, Gary S.
dc.contributor.authorMcGuire, P. M.
dc.date2022-08-11T08:10:56.000
dc.date.accessioned2022-08-23T17:25:57Z
dc.date.available2022-08-23T17:25:57Z
dc.date.issued1977-05-17
dc.date.submitted2011-01-14
dc.identifier.citationBiochemistry. 1977 May 17;16(10):2207-13.
dc.identifier.issn0006-2960 (Linking)
dc.identifier.pmid861206
dc.identifier.urihttp://hdl.handle.net/20.500.14038/49526
dc.description.abstractThe distribution of [3H]methyl radioactivity in cytoplasmic histone mRNA, isolated during the DNA synthetic (S) phase of the HeLa S3 cell cycle, has been investigated. Evidence is presented that approximately 30% of the radioactivity is in m7GpppXmpYp oligonucleotides, where Xm represents 2'-O-methylated adenosine and guanosine with a molar ratio of 4:1, respectively. The remainder of the radioactivity is present as m7GpppCmpYmpZp oligonucleotides, where Xm is again 2'-O-methylated adenosine and guanosine (4:1) and where ym represents 2'-O-methylated adenosine, guanosine, cytidine, and uridine with ratios of 2:1:1:1, respectively. While 48.6% of the [3H]methyl radioactivity was present as N6-methyladenosine in poly(adenylic acid)-terminated mRNA from S-phase cells, no evidence for N6-methyladenosine was found in histone mRNA. It thus appears that histone mRNA which lacks 3'-terminal poly(adenylic acid) sequences and functions on cytoplasmic polyribosomes during a limited protion of the cell cycle is capped but lacks internal-modified nucleosides.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=861206&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1021/bi00629a026
dc.subjectCell Division
dc.subjectDNA Replication
dc.subjectHela Cells
dc.subjectHistones
dc.subjectPoly A
dc.subject*RNA, Messenger
dc.subjectRibonucleosides
dc.subjectCell Biology
dc.titleHistone messenger RNA from HeLa cells: evidence for modified 5' termini
dc.typeJournal Article
dc.source.journaltitleBiochemistry
dc.source.volume16
dc.source.issue10
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/stein/191
dc.identifier.contextkey1728496
html.description.abstract<p>The distribution of [3H]methyl radioactivity in cytoplasmic histone mRNA, isolated during the DNA synthetic (S) phase of the HeLa S3 cell cycle, has been investigated. Evidence is presented that approximately 30% of the radioactivity is in m7GpppXmpYp oligonucleotides, where Xm represents 2'-O-methylated adenosine and guanosine with a molar ratio of 4:1, respectively. The remainder of the radioactivity is present as m7GpppCmpYmpZp oligonucleotides, where Xm is again 2'-O-methylated adenosine and guanosine (4:1) and where ym represents 2'-O-methylated adenosine, guanosine, cytidine, and uridine with ratios of 2:1:1:1, respectively. While 48.6% of the [3H]methyl radioactivity was present as N6-methyladenosine in poly(adenylic acid)-terminated mRNA from S-phase cells, no evidence for N6-methyladenosine was found in histone mRNA. It thus appears that histone mRNA which lacks 3'-terminal poly(adenylic acid) sequences and functions on cytoplasmic polyribosomes during a limited protion of the cell cycle is capped but lacks internal-modified nucleosides.</p>
dc.identifier.submissionpathstein/191
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages2207-13


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