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dc.contributor.authorStein, Janet L.
dc.contributor.authorReed, Keith A.
dc.contributor.authorStein, Gary S.
dc.date2022-08-11T08:10:56.000
dc.date.accessioned2022-08-23T17:25:58Z
dc.date.available2022-08-23T17:25:58Z
dc.date.issued1976-07-27
dc.date.submitted2011-01-14
dc.identifier.citationBiochemistry. 1976 Jul 27;15(15):3291-5.
dc.identifier.issn0006-2960 (Linking)
dc.identifier.pmid952856
dc.identifier.urihttp://hdl.handle.net/20.500.14038/49529
dc.description.abstractTo elucidate the manner in which histones and nonhistone chromosomal proteins interact to render histone genes transcribable in HeLa S3 cells, we have examined transcription of histone mRNA sequences from DNA, as well as from several DNA-chromosomal protein complexes. Histone mRNA sequences were assayed by hybridization to a 3H-labeled single-stranded DNA complementary to histone mRNAs. Our results indicate that DNA is an effective template for transcription of histone mRNA sequences and that histones by themselves inhibit transcription from DNA, including transcription of histone genes, in a dose-dependent, nonspecific manner. When complexed with DNA alone, nonhistone chromosomal proteins do not affect the transcription of histone mRNA sequences. However, when associated with DNA in the presence of histones, nonhistone chromosomal proteins are capable of selectively rendering histone genes transcribable. These results suggest a possible role for nonhistone chromosomal proteins in mediating the interactions of histones with DNA to render histone genes transcribable.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=952856&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1021/bi00660a019
dc.subjectBase Sequence
dc.subjectCell Division
dc.subjectChromatin
dc.subjectDNA, Neoplasm
dc.subjectDNA-Directed RNA Polymerases
dc.subjectHela Cells
dc.subjectHistones
dc.subjectNucleic Acid Hybridization
dc.subjectNucleoproteins
dc.subjectRNA, Messenger
dc.subjectTemplates, Genetic
dc.subject*Transcription, Genetic
dc.subjectCell Biology
dc.titleEffect of histones and nonhistone chromosomal proteins on the transcription of histone genes from HeLaS3 cell DNA
dc.typeJournal Article
dc.source.journaltitleBiochemistry
dc.source.volume15
dc.source.issue15
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/stein/196
dc.identifier.contextkey1728502
html.description.abstract<p>To elucidate the manner in which histones and nonhistone chromosomal proteins interact to render histone genes transcribable in HeLa S3 cells, we have examined transcription of histone mRNA sequences from DNA, as well as from several DNA-chromosomal protein complexes. Histone mRNA sequences were assayed by hybridization to a 3H-labeled single-stranded DNA complementary to histone mRNAs. Our results indicate that DNA is an effective template for transcription of histone mRNA sequences and that histones by themselves inhibit transcription from DNA, including transcription of histone genes, in a dose-dependent, nonspecific manner. When complexed with DNA alone, nonhistone chromosomal proteins do not affect the transcription of histone mRNA sequences. However, when associated with DNA in the presence of histones, nonhistone chromosomal proteins are capable of selectively rendering histone genes transcribable. These results suggest a possible role for nonhistone chromosomal proteins in mediating the interactions of histones with DNA to render histone genes transcribable.</p>
dc.identifier.submissionpathstein/196
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages3291-5


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