Show simple item record

dc.contributor.authorLi, Xin
dc.contributor.authorGibson, Gary
dc.contributor.authorKim, Jae-Sung
dc.contributor.authorKroin, Jeffrey S.
dc.contributor.authorXu, Shunbin
dc.contributor.authorVan Wijnen, Andre J.
dc.contributor.authorIm, Hee-Jeong
dc.date2022-08-11T08:10:57.000
dc.date.accessioned2022-08-23T17:26:14Z
dc.date.available2022-08-23T17:26:14Z
dc.date.issued2011-07-15
dc.date.submitted2012-05-10
dc.identifier.citationGene. 2011 Jul 1;480(1-2):34-41. Epub 2011 Mar 21. <a href="http://dx.doi.org/10.1016/j.gene.2011.03.003">Link to article on publisher's site</a>
dc.identifier.issn0378-1119 (Linking)
dc.identifier.doi10.1016/j.gene.2011.03.003
dc.identifier.pmid21397669
dc.identifier.urihttp://hdl.handle.net/20.500.14038/49592
dc.description.abstractBecause miR-146a is linked to osteoarthritis (OA) and cartilage degeneration is associated with pain, we have characterized the functional role of miR-146a in the regulation of human articular cartilage homeostasis and pain-related factors. Expression of miRNA 146a was analyzed in human articular cartilage and synovium, as well as in dorsal root ganglia (DRG) and spinal cord from a rat model for OA-related pain assessment. The functional effects of miR-146a on human chondrocytic, synovial, and microglia cells were studied in cells transfected with miR-146a. Using real-time PCR, we assessed the expression of chondrocyte metabolism-related genes in chondrocytes, genes for inflammatory factors in synovial cells, as well as pain-related proteins and ion channels in microglial cells. Previous studies showed that miR-146a is significantly upregulated in human peripheral knee OA joint tissues. Transfection of synthetic miR-146a significantly suppresses extracellular matrix-associated proteins (e.g., Aggrecan, MMP-13, ADAMTS-5, collagen II) in human knee joint chondrocytes and regulates inflammatory cytokines in synovial cells from human knee joints. In contrast, miR-146a is expressed at reduced levels in DRGs and dorsal horn of the spinal cords isolated from rats experiencing OA-induced pain. Exogenous supplementation of synthetic miR-146a significantly modulates inflammatory cytokines and pain-related molecules (e.g., TNFalpha, COX-2, iNOS, IL-6, IL8, RANTS and ion channel, TRPV1) in human glial cells. Our findings suggest that miR-146a controls knee joint homeostasis and OA-associated algesia by balancing inflammatory responses in cartilage and synovium with pain-related factors in glial cells. Hence, miR-146a may be useful for the treatment of both cartilage regeneration and pain symptoms caused by OA.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=21397669&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/j.gene.2011.03.003
dc.subjectAnimals
dc.subjectCartilage, Articular
dc.subjectCells, Cultured
dc.subjectChondrocytes
dc.subjectHumans
dc.subjectMicroRNAs
dc.subjectNeural Pathways
dc.subjectNeuroglia
dc.subjectOsteoarthritis
dc.subjectOsteoarthritis, Knee
dc.subjectPain
dc.subjectRats
dc.subjectSynovial Membrane
dc.subjectTransfection
dc.subjectCell Biology
dc.titleMicroRNA-146a is linked to pain-related pathophysiology of osteoarthritis
dc.typeJournal Article
dc.source.journaltitleGene
dc.source.volume480
dc.source.issue1-2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/stein/261
dc.identifier.contextkey2838653
html.description.abstract<p>Because miR-146a is linked to osteoarthritis (OA) and cartilage degeneration is associated with pain, we have characterized the functional role of miR-146a in the regulation of human articular cartilage homeostasis and pain-related factors. Expression of miRNA 146a was analyzed in human articular cartilage and synovium, as well as in dorsal root ganglia (DRG) and spinal cord from a rat model for OA-related pain assessment. The functional effects of miR-146a on human chondrocytic, synovial, and microglia cells were studied in cells transfected with miR-146a. Using real-time PCR, we assessed the expression of chondrocyte metabolism-related genes in chondrocytes, genes for inflammatory factors in synovial cells, as well as pain-related proteins and ion channels in microglial cells. Previous studies showed that miR-146a is significantly upregulated in human peripheral knee OA joint tissues. Transfection of synthetic miR-146a significantly suppresses extracellular matrix-associated proteins (e.g., Aggrecan, MMP-13, ADAMTS-5, collagen II) in human knee joint chondrocytes and regulates inflammatory cytokines in synovial cells from human knee joints. In contrast, miR-146a is expressed at reduced levels in DRGs and dorsal horn of the spinal cords isolated from rats experiencing OA-induced pain. Exogenous supplementation of synthetic miR-146a significantly modulates inflammatory cytokines and pain-related molecules (e.g., TNFalpha, COX-2, iNOS, IL-6, IL8, RANTS and ion channel, TRPV1) in human glial cells. Our findings suggest that miR-146a controls knee joint homeostasis and OA-associated algesia by balancing inflammatory responses in cartilage and synovium with pain-related factors in glial cells. Hence, miR-146a may be useful for the treatment of both cartilage regeneration and pain symptoms caused by OA.</p>
dc.identifier.submissionpathstein/261
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages34-41


Files in this item

Thumbnail
Name:
Publisher version

This item appears in the following Collection(s)

Show simple item record