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dc.contributor.authorBecker, Klaus A.
dc.contributor.authorStein, Janet L.
dc.contributor.authorLian, Jane B.
dc.contributor.authorVan Wijnen, Andre J.
dc.contributor.authorStein, Gary S.
dc.date2022-08-11T08:10:57.000
dc.date.accessioned2022-08-23T17:26:15Z
dc.date.available2022-08-23T17:26:15Z
dc.date.issued2010-01-24
dc.date.submitted2011-01-11
dc.identifier.citationJ Cell Physiol. 2010 Jan;222(1):103-10. <a href="http://dx.doi.org/10.1002/jcp.21925">Link to article on publisher's site</a>
dc.identifier.issn0021-9541 (Linking)
dc.identifier.doi10.1002/jcp.21925
dc.identifier.pmid19774559
dc.identifier.urihttp://hdl.handle.net/20.500.14038/49597
dc.description.abstractSelf-renewal of human embryonic stem (hES) cells proceeds by a unique abbreviated cell cycle with a shortened G1 phase and distinctions in molecular cell cycle regulatory parameters. In this study, we show that early lineage-commitment of pluripotent hES cells modifies cell cycle kinetics. Human ES cells acquire a lengthened G1 within 72 h after lineage-programming is initiated, as reflected by loss of the pluripotency factor Oct4 and alterations in nuclear morphology. In hES cells that maintain the pristine pluripotent state, we find that autocrine mechanisms contribute to sustaining the abbreviated cell cycle. Our data show that naive and mitotically synchronized pluripotent hES cells are competent to initiate two consecutive S phases in the absence of external growth factors. We conclude that short-term self-renewal of pluripotent hES cells occurs autonomously, in part due to secreted factors, and that pluripotency is functionally linked to the abbreviated hES cell cycle.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=19774559&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/jcp.21925
dc.subjectAnimals
dc.subjectAutocrine Communication
dc.subjectCell Count
dc.subjectCell Differentiation
dc.subjectCell Line
dc.subject*Cell Lineage
dc.subjectCell Proliferation
dc.subjectCulture Media, Conditioned
dc.subjectDNA
dc.subjectEmbryonic Stem Cells
dc.subject*G1 Phase
dc.subjectHumans
dc.subjectIntercellular Signaling Peptides and Proteins
dc.subjectMice
dc.subjectPluripotent Stem Cells
dc.subjectS Phase
dc.subjectSignal Transduction
dc.subjectCell Biology
dc.titleHuman embryonic stem cells are pre-mitotically committed to self-renewal and acquire a lengthened G1 phase upon lineage programming
dc.typeArticle
dc.source.journaltitleJournal of cellular physiology
dc.source.volume222
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/stein/27
dc.identifier.contextkey1724067
html.description.abstract<p>Self-renewal of human embryonic stem (hES) cells proceeds by a unique abbreviated cell cycle with a shortened G1 phase and distinctions in molecular cell cycle regulatory parameters. In this study, we show that early lineage-commitment of pluripotent hES cells modifies cell cycle kinetics. Human ES cells acquire a lengthened G1 within 72 h after lineage-programming is initiated, as reflected by loss of the pluripotency factor Oct4 and alterations in nuclear morphology. In hES cells that maintain the pristine pluripotent state, we find that autocrine mechanisms contribute to sustaining the abbreviated cell cycle. Our data show that naive and mitotically synchronized pluripotent hES cells are competent to initiate two consecutive S phases in the absence of external growth factors. We conclude that short-term self-renewal of pluripotent hES cells occurs autonomously, in part due to secreted factors, and that pluripotency is functionally linked to the abbreviated hES cell cycle.</p>
dc.identifier.submissionpathstein/27
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages103-10


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