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Subnuclear localization and intranuclear trafficking of transcription factors

dc.contributor.authorZaidi, Sayyed K.
dc.contributor.authorMedina, Ricardo F.
dc.contributor.authorPockwinse, Shirwin M.
dc.contributor.authorBakshi, Rachit
dc.contributor.authorKota, Krishna P.
dc.contributor.authorAli, Syed A.
dc.contributor.authorYoung, Daniel W.
dc.contributor.authorNickerson, Jeffrey A.
dc.contributor.authorJaved, Amjad
dc.contributor.authorMontecino, Martin A.
dc.contributor.authorVan Wijnen, Andre J.
dc.contributor.authorLian, Jane B.
dc.contributor.authorStein, Janet L.
dc.contributor.authorStein, Gary S.
dc.date2022-08-11T08:10:57.000
dc.date.accessioned2022-08-23T17:26:30Z
dc.date.available2022-08-23T17:26:30Z
dc.date.issued2010-08-10
dc.date.submitted2011-01-11
dc.identifier.citationMethods Mol Biol. 2010;647:77-93. <a href="http://dx.doi.org/10.1007/978-1-60761-738-9_4">Link to article on publisher's site</a>
dc.identifier.issn1064-3745 (Linking)
dc.identifier.doi10.1007/978-1-60761-738-9_4
dc.identifier.pmid20694661
dc.identifier.urihttp://hdl.handle.net/20.500.14038/49650
dc.description.abstractNuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=20694661&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1007/978-1-60761-738-9_4
dc.subjectIntranuclear Space
dc.subjectCell Biology
dc.titleSubnuclear localization and intranuclear trafficking of transcription factors
dc.typeJournal Article
dc.source.journaltitleMethods in molecular biology (Clifton, N.J.)
dc.source.volume647
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/stein/8
dc.identifier.contextkey1724048
html.description.abstract<p>Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.</p>
dc.identifier.submissionpathstein/8
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages77-93


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