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    Properties of blood-contacting surfaces of clinically implanted cardiac assist devices: gene expression, matrix composition, and ultrastructural characterization of cellular linings

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    Authors
    Menconi, Michael J.
    Pockwinse, Shirwin M.
    Owen, Thomas A.
    Dasse, Kurt A.
    Stein, Gary S.
    Lian, Jane B.
    UMass Chan Affiliations
    Department of Cell Biology
    Document Type
    Journal Article
    Publication Date
    1995-03-01
    Keywords
    Adult
    *Biocompatible Materials
    *Blood
    Case-Control Studies
    Cell Adhesion
    Evaluation Studies as Topic
    Female
    *Gene Expression
    *Heart-Assist Devices
    Humans
    Male
    Middle Aged
    Surface Properties
    Cell Biology
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    Link to Full Text
    http://dx.doi.org/10.1002/jcb.240570320
    Abstract
    The development of implantable cardiac assist devices for prolonged circulatory support has been impeded by the problem of excessive thrombogenesis on the blood-prosthetic interface, with subsequent embolization. To overcome this obstacle, a ventricular assist device has been developed with textured blood-contacting surfaces to encourage the formation of a tightly adherent, hemocompatible, biological lining. In this study, we applied molecular biological techniques, in conjunction with conventional ultrastructural and biochemical techniques, to characterize the biological linings associated with the blood-contacting surfaces of 11 of these devices, which had been clinically implanted for durations ranging from 21 to 324 days. No clinical thromboembolic events or pump-related thromboembolism occurred. Biological linings developed on the textured surfaces composed of patches of cellular tissue intermingled with areas of compact fibrinous material. In addition, islands of collagenous tissue containing fibroblast-like cells appeared after 30 days of implantation. Many of these cells contained microfilaments with dense bodies indicative of myofibroblasts. RNA hybridization analyses demonstrated that the colonizing cells actively expressed genes encoding proteins for cell proliferation (histones), adhesion (fibronectin), cytoskeleton (actin, vimentin) and extracellular matrix (types I and III collagen). Linings, which never exceeded 150 microns in thickness, remained free of pathological calcification. Textured blood-contacting surfaces induced the formation of a thin, tightly adherent, viable lining which exhibited excellent long-term hemocompatibility.
    Source
    J Cell Biochem. 1995 Mar;57(3):557-73. Link to article on publisher's site
    DOI
    10.1002/jcb.240570320
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/49653
    PubMed ID
    7768989
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1002/jcb.240570320
    Scopus Count
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