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dc.contributor.authorMenconi, Michael J.
dc.contributor.authorPockwinse, Shirwin M.
dc.contributor.authorOwen, Thomas A.
dc.contributor.authorDasse, Kurt A.
dc.contributor.authorStein, Gary S.
dc.contributor.authorLian, Jane B.
dc.date2022-08-11T08:10:57.000
dc.date.accessioned2022-08-23T17:26:30Z
dc.date.available2022-08-23T17:26:30Z
dc.date.issued1995-03-01
dc.date.submitted2011-01-11
dc.identifier.citationJ Cell Biochem. 1995 Mar;57(3):557-73. <a href="http://dx.doi.org/10.1002/jcb.240570320">Link to article on publisher's site</a>
dc.identifier.issn0730-2312 (Linking)
dc.identifier.doi10.1002/jcb.240570320
dc.identifier.pmid7768989
dc.identifier.urihttp://hdl.handle.net/20.500.14038/49653
dc.description.abstractThe development of implantable cardiac assist devices for prolonged circulatory support has been impeded by the problem of excessive thrombogenesis on the blood-prosthetic interface, with subsequent embolization. To overcome this obstacle, a ventricular assist device has been developed with textured blood-contacting surfaces to encourage the formation of a tightly adherent, hemocompatible, biological lining. In this study, we applied molecular biological techniques, in conjunction with conventional ultrastructural and biochemical techniques, to characterize the biological linings associated with the blood-contacting surfaces of 11 of these devices, which had been clinically implanted for durations ranging from 21 to 324 days. No clinical thromboembolic events or pump-related thromboembolism occurred. Biological linings developed on the textured surfaces composed of patches of cellular tissue intermingled with areas of compact fibrinous material. In addition, islands of collagenous tissue containing fibroblast-like cells appeared after 30 days of implantation. Many of these cells contained microfilaments with dense bodies indicative of myofibroblasts. RNA hybridization analyses demonstrated that the colonizing cells actively expressed genes encoding proteins for cell proliferation (histones), adhesion (fibronectin), cytoskeleton (actin, vimentin) and extracellular matrix (types I and III collagen). Linings, which never exceeded 150 microns in thickness, remained free of pathological calcification. Textured blood-contacting surfaces induced the formation of a thin, tightly adherent, viable lining which exhibited excellent long-term hemocompatibility.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=7768989&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/jcb.240570320
dc.subjectAdult
dc.subject*Biocompatible Materials
dc.subject*Blood
dc.subjectCase-Control Studies
dc.subjectCell Adhesion
dc.subjectEvaluation Studies as Topic
dc.subjectFemale
dc.subject*Gene Expression
dc.subject*Heart-Assist Devices
dc.subjectHumans
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectSurface Properties
dc.subjectCell Biology
dc.titleProperties of blood-contacting surfaces of clinically implanted cardiac assist devices: gene expression, matrix composition, and ultrastructural characterization of cellular linings
dc.typeJournal Article
dc.source.journaltitleJournal of cellular biochemistry
dc.source.volume57
dc.source.issue3
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/stein/82
dc.identifier.contextkey1724123
html.description.abstract<p>The development of implantable cardiac assist devices for prolonged circulatory support has been impeded by the problem of excessive thrombogenesis on the blood-prosthetic interface, with subsequent embolization. To overcome this obstacle, a ventricular assist device has been developed with textured blood-contacting surfaces to encourage the formation of a tightly adherent, hemocompatible, biological lining. In this study, we applied molecular biological techniques, in conjunction with conventional ultrastructural and biochemical techniques, to characterize the biological linings associated with the blood-contacting surfaces of 11 of these devices, which had been clinically implanted for durations ranging from 21 to 324 days. No clinical thromboembolic events or pump-related thromboembolism occurred. Biological linings developed on the textured surfaces composed of patches of cellular tissue intermingled with areas of compact fibrinous material. In addition, islands of collagenous tissue containing fibroblast-like cells appeared after 30 days of implantation. Many of these cells contained microfilaments with dense bodies indicative of myofibroblasts. RNA hybridization analyses demonstrated that the colonizing cells actively expressed genes encoding proteins for cell proliferation (histones), adhesion (fibronectin), cytoskeleton (actin, vimentin) and extracellular matrix (types I and III collagen). Linings, which never exceeded 150 microns in thickness, remained free of pathological calcification. Textured blood-contacting surfaces induced the formation of a thin, tightly adherent, viable lining which exhibited excellent long-term hemocompatibility.</p>
dc.identifier.submissionpathstein/82
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages557-73


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