Transcriptionally active nuclei isolated from intact bone reflect modified levels of gene expression in skeletal development and pathology
Jackson, Mary E.
Marks, Sandy C. Jr.
Stein, Janet L.
Lian, Jane B.
Stein, Gary S.
UMass Chan AffiliationsDepartment of Cell Biology
Bone and Bones
*Gene Expression Regulation
Promoter Regions, Genetic
Regulatory Sequences, Nucleic Acid
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AbstractTranscriptional regulation of gene expression in vivo in bone, associated with normal development or skeletal disorders, to date, has not been studied. We report the successful isolation of nuclei that are transcriptionally active from normal and osteopetrotic rat bone. Transcription rates of cell growth and bone-related genes (including histone H4, c-fos, c-jun, TGF beta 1, beta 2 macroglobulin, collagen, fibronectin, osteocalcin, osteopontin, and tartrate resistant acid phosphatase) change as a function of calvarial development from birth to 6 weeks and are selectively modified in osteopetrotic animals. Additionally, nuclei isolated from intact bone yield promoter binding factors. Bone nuclei, which transcribe faithfully and contain the normal complement of nuclear protein factors, offer a powerful approach for investigating in vivo gene regulation in skeletal development and pathology.
SourceJ Cell Biochem. 1994 Jun;55(2):182-9. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/49662
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