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    Transcriptionally active nuclei isolated from intact bone reflect modified levels of gene expression in skeletal development and pathology

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    Authors
    Shalhoub, Victoria
    Bortell, Rita
    Jackson, Mary E.
    Marks, Sandy C. Jr.
    Stein, Janet L.
    Lian, Jane B.
    Stein, Gary S.
    UMass Chan Affiliations
    Department of Cell Biology
    Document Type
    Journal Article
    Publication Date
    1994-06-01
    Keywords
    Animals
    Bone Development
    Bone and Bones
    Cell Differentiation
    Cell Nucleus
    Female
    *Gene Expression Regulation
    Male
    Osteoblasts
    Osteopetrosis
    Promoter Regions, Genetic
    RNA, Messenger
    Rats
    Regulatory Sequences, Nucleic Acid
    *Transcription, Genetic
    Cell Biology
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    Link to Full Text
    http://dx.doi.org/10.1002/jcb.240550205
    Abstract
    Transcriptional regulation of gene expression in vivo in bone, associated with normal development or skeletal disorders, to date, has not been studied. We report the successful isolation of nuclei that are transcriptionally active from normal and osteopetrotic rat bone. Transcription rates of cell growth and bone-related genes (including histone H4, c-fos, c-jun, TGF beta 1, beta 2 macroglobulin, collagen, fibronectin, osteocalcin, osteopontin, and tartrate resistant acid phosphatase) change as a function of calvarial development from birth to 6 weeks and are selectively modified in osteopetrotic animals. Additionally, nuclei isolated from intact bone yield promoter binding factors. Bone nuclei, which transcribe faithfully and contain the normal complement of nuclear protein factors, offer a powerful approach for investigating in vivo gene regulation in skeletal development and pathology.
    Source
    J Cell Biochem. 1994 Jun;55(2):182-9. Link to article on publisher's site
    DOI
    10.1002/jcb.240550205
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/49662
    PubMed ID
    8089193
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1002/jcb.240550205
    Scopus Count
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    UMass Chan Faculty and Researcher Publications

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