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dc.contributor.authorShalhoub, Victoria
dc.contributor.authorBortell, Rita
dc.contributor.authorJackson, Mary E.
dc.contributor.authorMarks, Sandy C. Jr.
dc.contributor.authorStein, Janet L.
dc.contributor.authorLian, Jane B.
dc.contributor.authorStein, Gary S.
dc.date2022-08-11T08:10:58.000
dc.date.accessioned2022-08-23T17:26:33Z
dc.date.available2022-08-23T17:26:33Z
dc.date.issued1994-06-01
dc.date.submitted2011-01-11
dc.identifier.citationJ Cell Biochem. 1994 Jun;55(2):182-9. <a href="http://dx.doi.org/10.1002/jcb.240550205">Link to article on publisher's site</a>
dc.identifier.issn0730-2312 (Linking)
dc.identifier.doi10.1002/jcb.240550205
dc.identifier.pmid8089193
dc.identifier.urihttp://hdl.handle.net/20.500.14038/49662
dc.description.abstractTranscriptional regulation of gene expression in vivo in bone, associated with normal development or skeletal disorders, to date, has not been studied. We report the successful isolation of nuclei that are transcriptionally active from normal and osteopetrotic rat bone. Transcription rates of cell growth and bone-related genes (including histone H4, c-fos, c-jun, TGF beta 1, beta 2 macroglobulin, collagen, fibronectin, osteocalcin, osteopontin, and tartrate resistant acid phosphatase) change as a function of calvarial development from birth to 6 weeks and are selectively modified in osteopetrotic animals. Additionally, nuclei isolated from intact bone yield promoter binding factors. Bone nuclei, which transcribe faithfully and contain the normal complement of nuclear protein factors, offer a powerful approach for investigating in vivo gene regulation in skeletal development and pathology.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=8089193&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/jcb.240550205
dc.subjectAnimals
dc.subjectBone Development
dc.subjectBone and Bones
dc.subjectCell Differentiation
dc.subjectCell Nucleus
dc.subjectFemale
dc.subject*Gene Expression Regulation
dc.subjectMale
dc.subjectOsteoblasts
dc.subjectOsteopetrosis
dc.subjectPromoter Regions, Genetic
dc.subjectRNA, Messenger
dc.subjectRats
dc.subjectRegulatory Sequences, Nucleic Acid
dc.subject*Transcription, Genetic
dc.subjectCell Biology
dc.titleTranscriptionally active nuclei isolated from intact bone reflect modified levels of gene expression in skeletal development and pathology
dc.typeArticle
dc.source.journaltitleJournal of cellular biochemistry
dc.source.volume55
dc.source.issue2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/stein/90
dc.identifier.contextkey1724132
html.description.abstract<p>Transcriptional regulation of gene expression in vivo in bone, associated with normal development or skeletal disorders, to date, has not been studied. We report the successful isolation of nuclei that are transcriptionally active from normal and osteopetrotic rat bone. Transcription rates of cell growth and bone-related genes (including histone H4, c-fos, c-jun, TGF beta 1, beta 2 macroglobulin, collagen, fibronectin, osteocalcin, osteopontin, and tartrate resistant acid phosphatase) change as a function of calvarial development from birth to 6 weeks and are selectively modified in osteopetrotic animals. Additionally, nuclei isolated from intact bone yield promoter binding factors. Bone nuclei, which transcribe faithfully and contain the normal complement of nuclear protein factors, offer a powerful approach for investigating in vivo gene regulation in skeletal development and pathology.</p>
dc.identifier.submissionpathstein/90
dc.contributor.departmentDepartment of Cell Biology
dc.source.pages182-9


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