SMC complexes differentially compact mitotic chromosomes according to genomic context
Authors
Schalbetter, Stephanie AndreaGoloborodko, Anton
Fudenberg, Geoffrey
Belton, Jon-Matthew
Miles, Catrina
Yu, Miao
Dekker, Job
Mirny, Leonid
Baxter, Jonathan
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyProgram in Systems Biology
Document Type
Journal ArticlePublication Date
2017-09-01Keywords
Chromosome condensationChromosome conformation capture-based methods
Cohesion
DNA sequencing
Mitosis
Computational Biology
Genomics
Structural Biology
Systems Biology
Metadata
Show full item recordAbstract
Structural maintenance of chromosomes (SMC) protein complexes are key determinants of chromosome conformation. Using Hi-C and polymer modelling, we study how cohesin and condensin, two deeply conserved SMC complexes, organize chromosomes in the budding yeast Saccharomyces cerevisiae. The canonical role of cohesin is to co-align sister chromatids, while condensin generally compacts mitotic chromosomes. We find strikingly different roles for the two complexes in budding yeast mitosis. First, cohesin is responsible for compacting mitotic chromosome arms, independently of sister chromatid cohesion. Polymer simulations demonstrate that this role can be fully accounted for through cis-looping of chromatin. Second, condensin is generally dispensable for compaction along chromosome arms. Instead, it plays a targeted role compacting the rDNA proximal regions and promoting resolution of peri-centromeric regions. Our results argue that the conserved mechanism of SMC complexes is to form chromatin loops and that distinct SMC-dependent looping activities are selectively deployed to appropriately compact chromosomes.Source
Nat Cell Biol. 2017 Sep;19(9):1071-1080. doi: 10.1038/ncb3594. Epub 2017 Aug 21. Link to article on publisher's site
DOI
10.1038/ncb3594Permanent Link to this Item
http://hdl.handle.net/20.500.14038/49843PubMed ID
28825700Related Resources
ae974a485f413a2113503eed53cd6c53
10.1038/ncb3594