C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2
AuthorsGao, Xin D.
Leszyk, John D.
Shaffer, Scott A.
Zhu, Lihua Julie
Wolfe, Scot A.
Sontheimer, Erik J.
UMass Chan AffiliationsProgram in Molecular Medicine
Department of Molecular, Cell and Cancer Biology
Program in Systems Biology
Department of Biochemistry and Molecular Pharmacology
Proteomics and Mass Spectrometry Facility
RNA Therapeutics Institute
Document TypeJournal Article
KeywordsAmino Acids, Peptides, and Proteins
Genetics and Genomics
MetadataShow full item record
AbstractMapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 biotinylation at genomic elements by restricted spatial tagging (C-BERST) allows the rapid, unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres and centromeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, thereby facilitating annotation of these factors and their roles.
Nat Methods. 2018 May 7. doi: 10.1038/s41592-018-0006-2. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/49853
The preprint version of this article as posted in bioRxiv is available.