C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2
Authors
Gao, Xin D.Tu, Li-Chun
Mir, Aamir
Ding, Yue-He
Leszyk, John D.
Dekker, Job
Shaffer, Scott A.
Zhu, Lihua Julie
Wolfe, Scot A.
Sontheimer, Erik J.
UMass Chan Affiliations
Program in Molecular MedicineDepartment of Molecular, Cell and Cancer Biology
Program in Systems Biology
Department of Biochemistry and Molecular Pharmacology
Proteomics and Mass Spectrometry Facility
RNA Therapeutics Institute
Document Type
Journal ArticlePublication Date
2018-05-07Keywords
Amino Acids, Peptides, and ProteinsBiochemistry
Genetics and Genomics
Molecular Biology
Structural Biology
Systems Biology
Metadata
Show full item recordAbstract
Mapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 biotinylation at genomic elements by restricted spatial tagging (C-BERST) allows the rapid, unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres and centromeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, thereby facilitating annotation of these factors and their roles.Source
Nat Methods. 2018 May 7. doi: 10.1038/s41592-018-0006-2. Link to article on publisher's site
DOI
10.1038/s41592-018-0006-2Permanent Link to this Item
http://hdl.handle.net/20.500.14038/49853PubMed ID
29735996Notes
The preprint version of this article as posted in bioRxiv is available.
Related Resources
ae974a485f413a2113503eed53cd6c53
10.1038/s41592-018-0006-2