Chromatin Immunoprecipitation (ChIP) Analysis of Protein-DNA Interactions
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyProgram in Systems Biology
Document Type
Journal ArticlePublication Date
2018-05-01
Metadata
Show full item recordAbstract
Here we describe chromatin immunoprecipitation (ChIP), a molecular approach that uses formaldehyde cross-linking to investigate genome structure and function. This approach allows us to determine the distribution of histone modifications (e.g., acetylation, methylation), the deposition of histone variants (H2AZ, H3.3, etc.), and the location of sequence-specific and general transcription factors. We introduce well-established ChIP-based methods that allow analysis of protein-DNA interactions in living cells.Source
Cold Spring Harb Protoc. 2018 May 1;2018(5):pdb.top082586. doi: 10.1101/pdb.top082586. Link to article on publisher's site
DOI
10.1101/pdb.top082586Permanent Link to this Item
http://hdl.handle.net/20.500.14038/49854PubMed ID
29717056Related Resources
ae974a485f413a2113503eed53cd6c53
10.1101/pdb.top082586