ChIP

Authors
Kim, Tae Hoon
Dekker, Job
UMass Chan Affiliations
Department of Biochemistry and Molecular Pharmacology
Program in Systems Biology
Document Type
Journal Article
Publication Date
2018-04-02
Keywords
Laboratory and Basic Science Research
Molecular Biology
Systems Biology

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Link to Full Text
https://doi.org/10.1101/pdb.prot082610
Abstract
ChIP is a powerful method for analyzing protein-DNA interactions that occur in vivo. A basic protocol involves immunoprecipitation of the cross-linked nucleoprotein complex and isolating and analyzing the DNA that is associated with the protein of interest. The antibody and chromatin amounts indicated here are for ChIP-chip and ChIP-seq and are approximately 10-fold higher than for a standard ChIP assay.
Source

Cold Spring Harb Protoc. 2018 Apr 2;2018(4):pdb.prot082610. doi: 10.1101/pdb.prot082610. Link to article on publisher's site
DOI
10.1101/pdb.prot082610
Permanent Link to this Item
http://hdl.handle.net/20.500.14038/49860
PubMed ID
29610359
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