High-Efficiency Yeast Transformation
| dc.contributor.author | Reece-Hoyes, John S. | |
| dc.contributor.author | Walhout, Albertha J M | |
| dc.date | 2022-08-11T08:10:59.000 | |
| dc.date.accessioned | 2022-08-23T17:27:28Z | |
| dc.date.available | 2022-08-23T17:27:28Z | |
| dc.date.issued | 2018-07-02 | |
| dc.date.submitted | 2018-09-19 | |
| dc.identifier.citation | <p>Cold Spring Harb Protoc. 2018 Jul 2;2018(7):pdb.prot094995. doi: 10.1101/pdb.prot094995.<a href="https://doi.org/10.1101/pdb.prot094995">Link to article on publisher's site</a></p> | |
| dc.identifier.issn | 1559-6095 (Linking) | |
| dc.identifier.doi | 10.1101/pdb.prot094995 | |
| dc.identifier.pmid | 29967274 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14038/49869 | |
| dc.description.abstract | High-efficiency yeast transformation is used for integrations into YM4271 (yeast one-hybrid (Y1H) DNA-bait generation), for transforming libraries of activation domain (AD)-prey clones into Y1H and yeast two-hybrid (Y2H)-bait strains, and for gap repair. The protocol takes 2 d to complete. | |
| dc.language.iso | en_US | |
| dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=29967274&dopt=Abstract">Link to Article in PubMed</a></p> | |
| dc.relation.url | https://doi.org/10.1101/pdb.prot094995 | |
| dc.subject | Genetics and Genomics | |
| dc.subject | Laboratory and Basic Science Research | |
| dc.subject | Systems Biology | |
| dc.title | High-Efficiency Yeast Transformation | |
| dc.type | Journal Article | |
| dc.source.journaltitle | Cold Spring Harbor protocols | |
| dc.source.volume | 2018 | |
| dc.source.issue | 7 | |
| dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/sysbio_pubs/141 | |
| dc.identifier.contextkey | 12900773 | |
| html.description.abstract | <p>High-efficiency yeast transformation is used for integrations into YM4271 (yeast one-hybrid (Y1H) DNA-bait generation), for transforming libraries of activation domain (AD)-prey clones into Y1H and yeast two-hybrid (Y2H)-bait strains, and for gap repair. The protocol takes 2 d to complete.</p> | |
| dc.identifier.submissionpath | sysbio_pubs/141 | |
| dc.contributor.department | Program in Molecular Medicine | |
| dc.contributor.department | Program in Systems Biology | |
| dc.source.pages | pdb.prot094995 |