UMass Chan AffiliationsProgram in Systems Biology
Department of Biochemistry and Molecular Pharmacology
Genetics and Genomics
Laboratory and Basic Science Research
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AbstractThis protocol describes the 5C method of detecting ligation products in 3C, ChIP-loop, or control libraries. The method starts with a purified 3C library, a purified ChIP-loop library, or a purified control library. Before starting the protocol, assess the quality of the library that will be used by performing semiquantitative polymerase chain reaction (PCR). Ligation products should be readily detectable and nearby restriction fragments should be ligated more frequently than distant fragments. The design of the 5C primers and methods to determine interaction frequencies are also described in this protocol.
Cold Spring Harb Protoc. 2018 Sep 4;2018(9):pdb.prot097899. doi: 10.1101/pdb.prot097899. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/49876