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dc.contributor.authorKim, Tae Hoon
dc.contributor.authorDekker, Job
dc.date2022-08-11T08:10:59.000
dc.date.accessioned2022-08-23T17:27:29Z
dc.date.available2022-08-23T17:27:29Z
dc.date.issued2018-09-04
dc.date.submitted2018-12-06
dc.identifier.citation<p>Cold Spring Harb Protoc. 2018 Sep 4;2018(9):pdb.prot097899. doi: 10.1101/pdb.prot097899. <a href="https://doi.org/10.1101/pdb.prot097899">Link to article on publisher's site</a></p>
dc.identifier.issn1559-6095 (Linking)
dc.identifier.doi10.1101/pdb.prot097899
dc.identifier.pmid30181223
dc.identifier.urihttp://hdl.handle.net/20.500.14038/49876
dc.description.abstractThis protocol describes the 5C method of detecting ligation products in 3C, ChIP-loop, or control libraries. The method starts with a purified 3C library, a purified ChIP-loop library, or a purified control library. Before starting the protocol, assess the quality of the library that will be used by performing semiquantitative polymerase chain reaction (PCR). Ligation products should be readily detectable and nearby restriction fragments should be ligated more frequently than distant fragments. The design of the 5C primers and methods to determine interaction frequencies are also described in this protocol.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=30181223&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1101/pdb.prot097899
dc.subjectBiochemistry
dc.subjectGenetic Phenomena
dc.subjectGenetics and Genomics
dc.subjectLaboratory and Basic Science Research
dc.subjectMolecular Biology
dc.subjectSystems Biology
dc.title5C Analysis of 3C, ChIP-Loop, and Control Libraries
dc.typeJournal Article
dc.source.journaltitleCold Spring Harbor protocols
dc.source.volume2018
dc.source.issue9
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/sysbio_pubs/148
dc.identifier.contextkey13437616
html.description.abstract<p>This protocol describes the 5C method of detecting ligation products in 3C, ChIP-loop, or control libraries. The method starts with a purified 3C library, a purified ChIP-loop library, or a purified control library. Before starting the protocol, assess the quality of the library that will be used by performing semiquantitative polymerase chain reaction (PCR). Ligation products should be readily detectable and nearby restriction fragments should be ligated more frequently than distant fragments. The design of the 5C primers and methods to determine interaction frequencies are also described in this protocol.</p>
dc.identifier.submissionpathsysbio_pubs/148
dc.contributor.departmentProgram in Systems Biology
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.source.pagespdb.prot097899


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