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dc.contributor.authorOudelaar, A. Marieke
dc.contributor.authorDavies, James O. J.
dc.contributor.authorHanssen, Lars L. P.
dc.contributor.authorTelenius, Jelena M.
dc.contributor.authorSchwessinger, Ron
dc.contributor.authorLiu, Yu
dc.contributor.authorBrown, Jill M.
dc.contributor.authorDownes, Damien J.
dc.contributor.authorChiariello, Andrea M.
dc.contributor.authorBianco, Simona
dc.contributor.authorNicodemi, Mario
dc.contributor.authorBuckle, Veronica J.
dc.contributor.authorDekker, Job
dc.contributor.authorHiggs, Douglas R.
dc.contributor.authorHughes, Jim R.
dc.date2022-08-11T08:10:59.000
dc.date.accessioned2022-08-23T17:27:31Z
dc.date.available2022-08-23T17:27:31Z
dc.date.issued2018-12-01
dc.date.submitted2018-12-06
dc.identifier.citation<p>Nat Genet. 2018 Dec;50(12):1744-1751. doi: 10.1038/s41588-018-0253-2. Epub 2018 Oct 29. <a href="https://doi.org/10.1038/s41588-018-0253-2">Link to article on publisher's site</a></p>
dc.identifier.issn1061-4036 (Linking)
dc.identifier.doi10.1038/s41588-018-0253-2
dc.identifier.pmid30374068
dc.identifier.urihttp://hdl.handle.net/20.500.14038/49881
dc.description.abstractThe promoters of mammalian genes are commonly regulated by multiple distal enhancers, which physically interact within discrete chromatin domains. How such domains form and how the regulatory elements within them interact in single cells is not understood. To address this we developed Tri-C, a new chromosome conformation capture (3C) approach, to characterize concurrent chromatin interactions at individual alleles. Analysis by Tri-C identifies heterogeneous patterns of single-allele interactions between CTCF boundary elements, indicating that the formation of chromatin domains likely results from a dynamic process. Within these domains, we observe specific higher-order structures that involve simultaneous interactions between multiple enhancers and promoters. Such regulatory hubs provide a structural basis for understanding how multiple cis-regulatory elements act together to establish robust regulation of gene expression.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=30374068&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1038/s41588-018-0253-2
dc.subjectAmino Acids, Peptides, and Proteins
dc.subjectCells
dc.subjectComputational Biology
dc.subjectGenetic Phenomena
dc.subjectMolecular Biology
dc.subjectStructural Biology
dc.subjectSystems Biology
dc.titleSingle-allele chromatin interactions identify regulatory hubs in dynamic compartmentalized domains
dc.typeJournal Article
dc.source.journaltitleNature genetics
dc.source.volume50
dc.source.issue12
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/sysbio_pubs/152
dc.identifier.contextkey13437626
html.description.abstract<p>The promoters of mammalian genes are commonly regulated by multiple distal enhancers, which physically interact within discrete chromatin domains. How such domains form and how the regulatory elements within them interact in single cells is not understood. To address this we developed Tri-C, a new chromosome conformation capture (3C) approach, to characterize concurrent chromatin interactions at individual alleles. Analysis by Tri-C identifies heterogeneous patterns of single-allele interactions between CTCF boundary elements, indicating that the formation of chromatin domains likely results from a dynamic process. Within these domains, we observe specific higher-order structures that involve simultaneous interactions between multiple enhancers and promoters. Such regulatory hubs provide a structural basis for understanding how multiple cis-regulatory elements act together to establish robust regulation of gene expression.</p>
dc.identifier.submissionpathsysbio_pubs/152
dc.contributor.departmentProgram in Systems Biology, Department of Biochemistry and Molecular Pharmacology
dc.source.pages1744-1751


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