A Delicate Balance between Bacterial Iron and Reactive Oxygen Species Supports Optimal C. elegans Development
Holdorf, Amy D.
Yilmaz, L. Safak
Walhout, Albertha J. M.
UMass Chan AffiliationsGraduate School of Biomedical Sciences
Program in Molecular Medicine
Program in Systems Biology
electron transport chain
flux balance analysis
metabolic network modeling
reactive oxygen species
Biochemical Phenomena, Metabolism, and Nutrition
Cellular and Molecular Physiology
Genetics and Genomics
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AbstractIron is an essential micronutrient for all forms of life; low levels of iron cause human disease, while too much iron is toxic. Low iron levels induce reactive oxygen species (ROS) by disruption of the heme and iron-sulfur cluster-dependent electron transport chain (ETC). To identify bacterial metabolites that affect development, we screened the Keio Escherichia coli collection and uncovered 244 gene deletion mutants that slow Caenorhabditis elegans development. Several of these genes encode members of the ETC cytochrome bo oxidase complex, as well as iron importers. Surprisingly, either iron or anti-oxidant supplementation reversed the developmental delay. This suggests that low bacterial iron results in high bacterial ROS and vice versa, which causes oxidative stress in C. elegans that subsequently impairs mitochondrial function and delays development. Our data indicate that the bacterial diets of C. elegans provide precisely tailored amounts of iron to support proper development.
Cell Host Microbe. 2019 Sep 11;26(3):400-411.e3. doi: 10.1016/j.chom.2019.07.010. Epub 2019 Aug 20. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/49885