Flexible ordering of antibody class switch and V(D)J joining during B-cell ontogeny
Authors
Kumar, SatyendraWuerffel, Robert
Achour, Ikbel
Lajoie, Bryan R.
Sen, Ranjan
Dekker, Job
Feeney, Ann J.
Kenter, Amy L.
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyPrograms in Systems Biology
Document Type
Journal ArticlePublication Date
2013-11-15Keywords
B-cell developmentAg gene rearrangement
Gene expression
Developmental Biology
Genetics and Genomics
Systems Biology
Metadata
Show full item recordAbstract
V(D)J joining is mediated by RAG recombinase during early B-lymphocyte development in the bone marrow (BM). Activation-induced deaminase initiates isotype switching in mature B cells of secondary lymphoid structures. Previous studies questioned the strict ontological partitioning of these processes. We show that pro-B cells undergo robust switching to a subset of immunoglobulin H (IgH) isotypes. Chromatin studies reveal that in pro-B cells, the spatial organization of the Igh locus may restrict switching to this subset of isotypes. We demonstrate that in the BM, V(D)J joining and switching are interchangeably inducible, providing an explanation for the hyper-IgE phenotype of Omenn syndrome.Source
Kumar S, Wuerffel R, Achour I, Lajoie B, Sen R, Dekker J, Feeney AJ, Kenter AL. Flexible ordering of antibody class switch and V(D)J joining during B-cell ontogeny. Genes Dev. 2013 Nov 15;27(22):2439-44. doi: 10.1101/gad.227165.113. Link to article on publisher's siteDOI
10.1101/gad.227165.113Permanent Link to this Item
http://hdl.handle.net/20.500.14038/49920PubMed ID
24240234Notes
© 2013 Kumar et al.; Published by Cold Spring Harbor Laboratory Press.
This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.
Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1101/gad.227165.113