Flexible ordering of antibody class switch and V(D)J joining during B-cell ontogeny
Lajoie, Bryan R.
Feeney, Ann J.
Kenter, Amy L.
UMass Chan AffiliationsDepartment of Biochemistry and Molecular Pharmacology
Programs in Systems Biology
Document TypeJournal Article
Ag gene rearrangement
Genetics and Genomics
MetadataShow full item record
AbstractV(D)J joining is mediated by RAG recombinase during early B-lymphocyte development in the bone marrow (BM). Activation-induced deaminase initiates isotype switching in mature B cells of secondary lymphoid structures. Previous studies questioned the strict ontological partitioning of these processes. We show that pro-B cells undergo robust switching to a subset of immunoglobulin H (IgH) isotypes. Chromatin studies reveal that in pro-B cells, the spatial organization of the Igh locus may restrict switching to this subset of isotypes. We demonstrate that in the BM, V(D)J joining and switching are interchangeably inducible, providing an explanation for the hyper-IgE phenotype of Omenn syndrome.
SourceKumar S, Wuerffel R, Achour I, Lajoie B, Sen R, Dekker J, Feeney AJ, Kenter AL. Flexible ordering of antibody class switch and V(D)J joining during B-cell ontogeny. Genes Dev. 2013 Nov 15;27(22):2439-44. doi: 10.1101/gad.227165.113. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/49920
© 2013 Kumar et al.; Published by Cold Spring Harbor Laboratory Press.
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