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Flexible ordering of antibody class switch and V(D)J joining during B-cell ontogeny

dc.contributor.authorKumar, Satyendra
dc.contributor.authorWuerffel, Robert
dc.contributor.authorAchour, Ikbel
dc.contributor.authorLajoie, Bryan R.
dc.contributor.authorSen, Ranjan
dc.contributor.authorDekker, Job
dc.contributor.authorFeeney, Ann J.
dc.contributor.authorKenter, Amy L.
dc.date2022-08-11T08:11:00.000
dc.date.accessioned2022-08-23T17:27:41Z
dc.date.available2022-08-23T17:27:41Z
dc.date.issued2013-11-15
dc.date.submitted2013-12-04
dc.identifier.citationKumar S, Wuerffel R, Achour I, Lajoie B, Sen R, Dekker J, Feeney AJ, Kenter AL. Flexible ordering of antibody class switch and V(D)J joining during B-cell ontogeny. Genes Dev. 2013 Nov 15;27(22):2439-44. doi: 10.1101/gad.227165.113. <a href="http://dx.doi.org/10.1101/gad.227165.113">Link to article on publisher's site</a>
dc.identifier.issn0890-9369 (Linking)
dc.identifier.doi10.1101/gad.227165.113
dc.identifier.pmid24240234
dc.identifier.urihttp://hdl.handle.net/20.500.14038/49920
dc.description<p>© 2013 Kumar et al.; Published by Cold Spring Harbor Laboratory Press.</p> <p id="x-x-x-x-x-x-p-3">This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.</p>
dc.description.abstractV(D)J joining is mediated by RAG recombinase during early B-lymphocyte development in the bone marrow (BM). Activation-induced deaminase initiates isotype switching in mature B cells of secondary lymphoid structures. Previous studies questioned the strict ontological partitioning of these processes. We show that pro-B cells undergo robust switching to a subset of immunoglobulin H (IgH) isotypes. Chromatin studies reveal that in pro-B cells, the spatial organization of the Igh locus may restrict switching to this subset of isotypes. We demonstrate that in the BM, V(D)J joining and switching are interchangeably inducible, providing an explanation for the hyper-IgE phenotype of Omenn syndrome.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=24240234&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1101/gad.227165.113
dc.subjectB-cell development
dc.subjectAg gene rearrangement
dc.subjectGene expression
dc.subjectDevelopmental Biology
dc.subjectGenetics and Genomics
dc.subjectSystems Biology
dc.titleFlexible ordering of antibody class switch and V(D)J joining during B-cell ontogeny
dc.typeJournal Article
dc.source.journaltitleGenes and development
dc.source.volume27
dc.source.issue22
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1036&amp;context=sysbio_pubs&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/sysbio_pubs/37
dc.identifier.contextkey4886223
refterms.dateFOA2022-08-23T17:27:42Z
html.description.abstract<p>V(D)J joining is mediated by RAG recombinase during early B-lymphocyte development in the bone marrow (BM). Activation-induced deaminase initiates isotype switching in mature B cells of secondary lymphoid structures. Previous studies questioned the strict ontological partitioning of these processes. We show that pro-B cells undergo robust switching to a subset of immunoglobulin H (IgH) isotypes. Chromatin studies reveal that in pro-B cells, the spatial organization of the Igh locus may restrict switching to this subset of isotypes. We demonstrate that in the BM, V(D)J joining and switching are interchangeably inducible, providing an explanation for the hyper-IgE phenotype of Omenn syndrome.</p>
dc.identifier.submissionpathsysbio_pubs/37
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.contributor.departmentPrograms in Systems Biology
dc.source.pages2439-44


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