UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyProgram in Systems Biology
Document Type
Journal ArticlePublication Date
2015-06-01Keywords
Biochemistry, Biophysics, and Structural BiologyGenetics and Genomics
Laboratory and Basic Science Research
Systems Biology
Metadata
Show full item recordAbstract
In experiments using chromosome conformation capture followed by PCR (3C-PCR) or chromosome conformation capture carbon copy (5C), it is critical to control for intrinsic biases in the restriction fragments of interest and the probes or primers used for detection. Characteristics such as GC%, annealing temperature, efficiency of 3C primers or 5C probes, and length of restriction fragment can cause variations in primer or probe performance and fragment ligation efficiency. Bias can be measured empirically by production of a random control library, as described here, to be used with the 3C library of interest.Source
Cold Spring Harb Protoc. 2015 Jun 1;2015(6):587-92. doi: 10.1101/pdb.prot085183. Link to article on publisher's siteDOI
10.1101/pdb.prot085183Permanent Link to this Item
http://hdl.handle.net/20.500.14038/49951PubMed ID
26034305Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1101/pdb.prot085183