Genome-wide Maps of Nuclear Lamina Interactions in Single Human Cells
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Authors
Kind, JopPagie, Ludo
de Vries, Sandra S.
Nahidiazar, Leila
Dey, Siddharth S.
Bienko, Magda
Zhan, Ye
Lajoie, Bryan
de Graaf, Carolyn A.
Amendola, Mario
Fudenberg, Geoffrey
Imakaev, Maxim
Mirny, Leonid A.
Jalink, Kees
Dekker, Job
van Oudenaarden, Alexander
van Steensel, Bas
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyProgram in Systems Biology
Document Type
Journal ArticlePublication Date
2015-09-24
Metadata
Show full item recordAbstract
Mammalian interphase chromosomes interact with the nuclear lamina (NL) through hundreds of large lamina-associated domains (LADs). We report a method to map NL contacts genome-wide in single human cells. Analysis of nearly 400 maps reveals a core architecture consisting of gene-poor LADs that contact the NL with high cell-to-cell consistency, interspersed by LADs with more variable NL interactions. The variable contacts tend to be cell-type specific and are more sensitive to changes in genome ploidy than the consistent contacts. Single-cell maps indicate that NL contacts involve multivalent interactions over hundreds of kilobases. Moreover, we observe extensive intra-chromosomal coordination of NL contacts, even over tens of megabases. Such coordinated loci exhibit preferential interactions as detected by Hi-C. Finally, the consistency of NL contacts is inversely linked to gene activity in single cells and correlates positively with the heterochromatic histone modification H3K9me3. These results highlight fundamental principles of single-cell chromatin organization.Source
Cell. 2015 Sep 24;163(1):134-47. doi: 10.1016/j.cell.2015.08.040. Epub 2015 Sep 10. Link to article on publisher's siteDOI
10.1016/j.cell.2015.08.040Permanent Link to this Item
http://hdl.handle.net/20.500.14038/49957PubMed ID
26365489Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/j.cell.2015.08.040