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    Genome-wide Maps of Nuclear Lamina Interactions in Single Human Cells

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    Authors
    Kind, Jop
    Pagie, Ludo
    de Vries, Sandra S.
    Nahidiazar, Leila
    Dey, Siddharth S.
    Bienko, Magda
    Zhan, Ye
    Lajoie, Bryan
    de Graaf, Carolyn A.
    Amendola, Mario
    Fudenberg, Geoffrey
    Imakaev, Maxim
    Mirny, Leonid A.
    Jalink, Kees
    Dekker, Job
    van Oudenaarden, Alexander
    van Steensel, Bas
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    UMass Chan Affiliations
    Department of Biochemistry and Molecular Pharmacology
    Program in Systems Biology
    Document Type
    Journal Article
    Publication Date
    2015-09-24
    Keywords
    Genomics
    Molecular Biology
    Systems Biology
    
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    Link to Full Text
    http://dx.doi.org/10.1016/j.cell.2015.08.040
    Abstract
    Mammalian interphase chromosomes interact with the nuclear lamina (NL) through hundreds of large lamina-associated domains (LADs). We report a method to map NL contacts genome-wide in single human cells. Analysis of nearly 400 maps reveals a core architecture consisting of gene-poor LADs that contact the NL with high cell-to-cell consistency, interspersed by LADs with more variable NL interactions. The variable contacts tend to be cell-type specific and are more sensitive to changes in genome ploidy than the consistent contacts. Single-cell maps indicate that NL contacts involve multivalent interactions over hundreds of kilobases. Moreover, we observe extensive intra-chromosomal coordination of NL contacts, even over tens of megabases. Such coordinated loci exhibit preferential interactions as detected by Hi-C. Finally, the consistency of NL contacts is inversely linked to gene activity in single cells and correlates positively with the heterochromatic histone modification H3K9me3. These results highlight fundamental principles of single-cell chromatin organization.
    Source
    Cell. 2015 Sep 24;163(1):134-47. doi: 10.1016/j.cell.2015.08.040. Epub 2015 Sep 10. Link to article on publisher's site
    DOI
    10.1016/j.cell.2015.08.040
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/49957
    PubMed ID
    26365489
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.cell.2015.08.040
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